Starch Content Assay Kit
淀粉含量检测试剂盒
货号:AKSU015C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Starch Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    浓硫酸(H2SO4,MW = 98.08,CAS: 7664-93-9)
Detection Principle
使用洗脱液将样品中可溶性糖与淀粉分开,淀粉经酸水解为葡萄糖,进一步与蒽酮反应生成蓝绿色糠醛衍生物,产物在620 nm处具有特征吸收峰,通过吸光值变化即可定量检测淀粉的含量。
  • 检测方法: 蒽酮比色法
  • 检测波长: 620 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=8.3736x+0.0073 (R2=0.9998)
  • 标准线性范围: 0.01-0.12 mg/mL
  • 检测限: 0.003 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

②浓硫酸具有强腐蚀性,请做好防护措施并谨慎操作;

③待测样本稀释倍数可参照公式计算

最大稀释倍数(DMax)=74"×W×A"

最小稀释倍数(DMin)=7.4"×W×A"

注释:W:样品质量,g;A:预估淀粉含量,mg/g;例:预估淀粉含量为400 mg/g(40%),称取0.05 g样本进行淀粉提取,DMin=148,DMax=1480,即待测样本稀释倍数可选择150-1400倍。

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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[2] Zhao Y, Yu S, Tan J, et al. Bioconversion of citrus waste by long-term DMSO-cryopreserved rumen fluid to volatile fatty acids and biogas is feasible: A microbiome perspective[J]. Journal of Environmental Management, 2024, 351: 119693.(IF 8.7)

[3] Zhang Y, Dai T, Liu Y, et al. Effect of exogenous glycine betaine on the germination of tomato seeds under cold stress[J]. International Journal of Molecular Sciences, 2022, 23(18): 10474.(IF 6.208)

[4] Peng D, Tang D, Zhong C, et al. Interactions between Fuzi (Aconiti Lateralis Radix Preparata) total alkaloids and Fuzi starch: Structural, physicochemical, and rheological properties[J]. LWT, 2023, 182: 114879.(IF 6)

[5] Mao J, Gao Z, Lin M, et al. Targeted multi-platform metabolome analysis and enzyme activity analysis of kiwifruit during postharvest ripening[J]. Frontiers in Plant Science, 2023, 14: 1120166.(IF 5.6)

[6] Dong L, Wang F, Chen L, et al. Metabolomic analysis reveals the responses of docosahexaenoic-acid-producing Schizochytrium under hyposalinity conditions[J]. Algal Research, 2023, 70: 102987.(IF 5.1)

[7] Wang W, An C, Yao Y, et al. De novo biosynthesis and gram-level production of m-cresol in Aspergillus nidulans[J]. Applied Microbiology and Biotechnology, 2021, 105: 6333-6343.(IF 4.813)

[8] Yu Z P, An C, Yao Y, et al. A combined strategy for the overproduction of complex ergot alkaloid agroclavine[J]. Synthetic and Systems Biotechnology, 2022, 7(4): 1126-1132.(IF 4.692)

[9] Wei F, Ma N, Haseeb H A, et al. Insights into structural and physicochemical properties of maize starch after Fusarium verticillioides infection[J]. Journal of Food Composition and Analysis, 2022, 114: 104819.(IF 4.52)

[10] Wan X, Yao G, Wang K, et al. Transcriptomic Analysis of the Response of the Toxic Dinoflagellate Prorocentrum lima to Phosphorous Limitation[J]. Microorganisms, 2023, 11(9): 2216.(IF 4.5)

[11] Li Z, Li X, He F. Non-structural carbohydrates contributed to cold tolerance and regeneration of Medicago sativa L[J]. Planta, 2023, 257(6): 116.(IF 4.3)

[12] Li X, Shen D, Mao J, et al. Increased weight loss and internal air space, degraded starch and pectin combined to cause pulp mealiness in ‘Oregon Spur Ⅱ’apples during ambient storage[J]. Scientia Horticulturae, 2024, 324: 112629.(IF 4.3)

[13] Li X, Li Y, Xi R, et al. GWAS identifies candidate genes affecting water absorption in foxtail millet seeds[J]. Plant Growth Regulation, 2023: 1-9.(IF 4.2)

[14] Ren F, Liu M, Liu Y, et al. Core microbes closely related with the nutrients and flavor of sweet fermented oats (whole grain food) from China[J]. World Journal of Microbiology and Biotechnology, 2023, 39(9): 236.(IF 4.1)

[15] Zhang G, Hua D, Xu J, et al. Pulsed light treatment enhances starch hydrolysis and improves starch physicochemical properties of germinated brown rice[J]. Journal of the Science of Food and Agriculture, 2023.(IF 4.1)

[16] Li Z, Li X, He F. Drip Irrigation Depth Alters Root Morphology and Architecture and Cold Resistance of Alfalfa[J]. Agronomy, 2022, 12(9): 2192.(IF 3.949)

[17] Li M, Xu F, Zhao Y, et al. High-Efficient Production of Cellulosic Ethanol from Corn Fiber Based on the Suitable C5/C6 Co-Fermentation Saccharomyces cerevisiae Strain[J]. Fermentation, 2023, 9(8): 743.(IF 3.7)

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