Themicellulose Content Assay Kit
半纤维素含量检测试剂盒
货号:AKSU008C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Themicellulose Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
半纤维素经酸处理后转化成还原糖,进一步与3,5-二硝基水杨酸反应生成棕红色氨基化合物,产物在540 nm处具有特征吸收峰,通过吸光值的变化即可定量检测半纤维素的含量。
  • 检测方法: DNS比色法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: D-Xylose
  • 参考标准: y=1.6986x-0.1086 (R2=0.9999)
  • 标准线性范围: 0.1-1.0 mg/mL
  • 检测限: 0.08 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准线性吸光值范围:高于最高值建议将上清液适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Huang Q, Hong T, Zheng M, et al. High-pressure homogenization treatment of red seaweed Bangia fusco-purpurea affects the physicochemical, functional properties and enhances in vitro anti-glycation activity of its dietary fibers[J]. Innovative Food Science & Emerging Technologies, 2023, 86: 103369.(IF 6.6)

[2] Jiang C, Wang R, Liu X, et al. Effect of particle size on physicochemical properties and in vitro hypoglycemic ability of insoluble dietary fiber from corn bran[J]. Frontiers in Nutrition, 2022, 9: 951821.(IF 6.59)

[3] Liu Z, Hu Y, Du A, et al. Cell Wall Matrix Polysaccharides Contribute to Salt–Alkali Tolerance in Rice[J]. International Journal of Molecular Sciences, 2022, 23(23): 15019.(IF 6.208)

[4] Ouyang H, Wu L, Hu Y, et al. Effect of steam explosion treatment on physicochemical, functional and structural properties of pomelo fruitlets[J]. LWT, 2023: 114963.(IF 6)

[5] Guo F, Yu W, Fu F, et al. Physiological, transcriptome and metabolome analyses provide molecular insights to seasonal development in Ginkgo biloba xylem[J]. Industrial Crops and Products, 2024, 208: 117930.(IF 5.9)

[6] Zhang M, Zhou Y, Yang X, et al. Preparation of esterified biomass waste hydrogels and their removal of Pb2+, Cu2+ and Cd2+ from aqueous solution[J]. Environmental Science and Pollution Research, 2023, 30(19): 56580-56593.(IF 5.8)

[7] Sun W, Chen Y, Zeng J, et al. The Tartary buckwheat bHLH gene ALCATRAZ contributes to silique dehiscence in Arabidopsis thaliana[J]. Plant Science, 2023, 333: 111733.(IF 5.2)

[8] Huang Y, Li Y, Zou K, et al. The Resistance of Maize to Ustilago maydis Infection Is Correlated with the Degree of Methyl Esterification of Pectin in the Cell Wall[J]. International Journal of Molecular Sciences, 2023, 24(19): 14737.(IF 5.6)

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