Reducing Sugar Content Assay Kit
还原糖含量检测试剂盒
货号:AKSU002M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Reducing Sugar Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
还原糖在碱性溶液中能够与3,5-二硝基水杨酸反应生成棕红色氨基化合物,产物在540 nm处具有特征吸收峰,还原糖在一定的浓度范围内与540 nm处吸光值呈线性关系,根据吸光值变化即可定量检测还原糖的含量。
  • 检测方法: DNS比色法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=4.1327x-0.1664 (R2=0.9997)
  • 标准线性范围: 0.05-0.4 mg/mL
  • 检测限: 0.05 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若A测定或ΔA测定超出标准吸光值线性范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定;低于最低值建议制备更高浓度样本后再进行测定,计算时相应修改;

②95℃处理5 min显色后溶液若出现浑浊,建议10000 g常温离心10 min,取上清检测吸光值;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhi J, Liu X, Xu Y, et al. Metal ion-mediated modulation of morphology, physicochemical properties, and digestibility of type 3 resistant starch microparticle[J]. Carbohydrate Polymers, 2023, 316: 121027.(IF 11.2)

[2]Zhu X, Ma S, Huang S, et al. Directed self-assembly of mesoporous starch-based microparticle: Characterization, antimicrobial activities, and digestibility[J]. Food Hydrocolloids, 2024: 110271. (IF 11.0)

[3] Adra H J, Zhi J, Luo K, et al. Facile preparation of highly uniform type 3 resistant starch nanoparticles[J]. Carbohydrate Polymers, 2022, 294: 119842.(IF 10.723)

[4] Huang S, Zhu X, Ma S, et al. Tween 80-mediated size-controlled self-assembly of resistant starch particle: A green and biodegradable white pigment for food applications[J]. Food Hydrocolloids, 2024, 153: 109993. (IF 10.7)

[5] Luo K, Kim Y R. Modulation of the self-assembly kinetics and digestibility of type 3 resistant starch particles by co-crystallization with amino acid[J]. Food Chemistry, 2023, 419: 136008.(IF 8.8)

[6] Zhu H, Chen Z, Zhang K, et al. The key role of sugar metabolism in the dormancy release and bud development of Lilium brownii var. viridulum Baker bulbs[J]. Scientia Horticulturae, 2024, 336: 113453. (IF 3.9)

[7] Huang L, Han Y, Zhong K, et al. Exploring the fungal communities and their correlation with the physicochemical properties of Zaopocu, a distinctive fermented food on Hainan island[J]. Biologia, 2023: 1-18.(IF 1.5)

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