D-Glucose Content Assay Kit (GOPOD Format)
葡萄糖含量检测试剂盒
货号:AKSU001C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
D-Glucose Content Assay Kit (GOPOD Format)
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:2 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
葡萄糖氧化酶(Glucose Oxidase,GOD)能够将葡萄糖氧化为葡萄糖酸,并释放H₂O₂;过氧化物酶(Peroxidase,POD)催化H₂O₂氧化4-氨基安替比林偶联酚,生成红色醌类化合物,产物在505 nm处具有特征吸收峰,通过吸光值变化即可定量检测葡萄糖的含量。
  • 检测方法: GOPOD Format
  • 检测波长: 505 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=3.7126x-0.0098 (R²=0.9997)
  • 标准线性范围: 0.025-0.4 mg/mL
  • 检测限: 0.002 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂一和试剂二避免与皮肤接触,若不慎溢出请用大量水冲洗接触部位;

②若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] X Li, X Huang, Mo J , et al. A Fully Integrated Closed-Loop System Based on Mesoporous Microneedles-Iontophoresis for Diabetes Treatment[J]. Advanced Science, 2021.(IF 16.806)

[2] Zhu Y, Jing L, X Li, et al. Decabromodiphenyl ethane disturbs hepatic glycolipid metabolism by regulating the PI3K/AKT/GLUT4 and mTOR/PPARγ/RXRα pathway in mice and L02 cells[J]. Science of The Total Environment, 2020, 763(7):142936.(IF 7.963)

[3] Yu M, Zhang L, Sun S , et al. Gliquidone improves retinal injury to relieve diabetic retinopathy via regulation of SIRT1/Notch1 pathway[J]. BMC Ophthalmology, 2021, 21(1).(IF 2.209)

[4] Wang C, Li X, Zhang W, et al. ETNPPL impairs autophagy through regulation of the ARG2-ROS signaling axis, contributing to palmitic acid-induced hepatic insulin resistance[J]. Free Radical Biology and Medicine, 2023. (IF 8.101)

[5] Chen X, Liu P, Zhang W, et al. ETNPPL modulates hyperinsulinemia‐induced insulin resistance through the SIK1/ROS‐mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes[J]. Journal of Cellular Physiology, 2023. (IF 6.51)

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