Trehalose Content Assay Kit
海藻糖含量检测试剂盒
货号:AKSU005C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Trehalose Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    浓硫酸(H2SO4,MW=98.078,CAS: 7664-93-9)
Detection Principle
海藻糖在强酸性条件下脱水生成5-羟甲基糠醛,能够与蒽酮反应生成蓝绿色糠醛衍生物,产物在620 nm处具有特征吸收峰,通过吸光值变化即可定量检测海藻糖的含量。
  • 检测方法: 蒽酮比色法
  • 检测波长: 620 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Trehalose
  • 参考标准: y=9.1184x+0.0043(R2=0.9998)
  • 标准线性范围: 0.005-0.10 mg/mL
  • 检测限: 0.002 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若A测定超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定;低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

②浓硫酸具有强腐蚀性,操作时需特别防护;

③本产品仅可用于除海藻糖外不含其他可溶性糖样本的测定;④待测样本不能用于蛋白浓度测定,组织样本需使用PBS单独提取后再进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zong S, Yun H, Yu X, et al. Bioaugmentation of Cd (II) removal in high-salinity wastewater by engineered Escherichia coli harbouring EC20 and irrE genes[J]. Journal of Cleaner Production, 2023: 137656.(IF 11.1)

[2] Liu T, Wang J, Chen L, et al. ScAREB4 promotes potato constitutive and acclimated freezing tolerance associated with enhancing trehalose synthesis and oxidative stress tolerance[J]. Plant, Cell & Environment, 2023, 46(12): 3839-3857.(IF 7.3)

[3] Li Z, Li X, He F. Drip Irrigation Depth Alters Root Morphology and Architecture and Cold Resistance of Alfalfa[J]. Agronomy, 2022, 12(9): 2192.(IF 3.949)

[4] Huo Y, Song Z, Wang H, et al. GrpE is involved in mitochondrial function and is an effective target for RNAi‐mediated pest and arbovirus control[J]. Insect Molecular Biology, 2022, 31(3): 377-390.(IF 3.424)

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