Lignin Content Assay Kit
木质素含量检测试剂盒
货号:AKSU010M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Lignin Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96 孔UV板
  • 预计测定时间:3 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    高氯酸(HClO4,MW = 100.46,CAS: 7601-90-3);
    冰乙酸(C2H4O2,MW = 60.05,CAS: 64-19-7)
Detection Principle
木质素中的酚羟基发生乙酰化后生成乙酰木质素,产物在280 nm处具有特征吸收峰,通过吸光值变化即可定量检测木质素的含量。
  • 检测方法: 乙酰化法
  • 检测波长: 280 nm
  • 信号响应: 递增型
Notices

①应适当调整冰乙酸加入量,使A测定处于0.1-0.8之间可提高结果准确性,计算时相应修改;

②试剂一和冰乙酸具有刺激性和挥发性,高氯酸具有强腐蚀性,操作时请做好防护措施;

③加热过程需严格密封,以防气体溢出,加热过程伴随有剧烈反应,混匀时应缓慢轻摇,以免压力过大喷出造成伤害;

④若玻璃试管操作不方便,可使用带盖密封性较好的离心管作为反应容器,请确保无橡胶等易腐蚀材质,以免造成泄漏;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Li H, Yu K, Zhang Z, et al. Targeted mutagenesis of flavonoid biosynthesis pathway genes reveals functional divergence in seed coat colour, oil content and fatty acid composition in Brassica napus L[J]. Plant Biotechnology Journal, 2023.(IF 13.8)

[2] Yao X, Li H, Nie J, et al. Disruption of the amino acid transporter CsAAP2 inhibits auxin‐mediated root development in cucumber[J]. New Phytologist, 2023.(IF 9.4)

[3] Kong L, Song Q, Wei H, et al. The AP2/ERF transcription factor PtoERF15 confers drought tolerance via JA‐mediated signaling in Populus[J]. New Phytologist, 2023, 240(5): 1848-1867.(IF 9.4)

[4] Huang Q, Hong T, Zheng M, et al. High-pressure homogenization treatment of red seaweed Bangia fusco-purpurea affects the physicochemical, functional properties and enhances in vitro anti-glycation activity of its dietary fibers[J]. Innovative Food Science & Emerging Technologies, 2023, 86: 103369.(IF 6.6)

[5] Jiang C, Wang R, Liu X, et al. Effect of particle size on physicochemical properties and in vitro hypoglycemic ability of insoluble dietary fiber from corn bran[J]. Frontiers in Nutrition, 2022, 9: 951821.(IF 6.59)

[6] Jiang M, Yu N, Zhang Y, et al. Deletion of Diterpenoid Biosynthetic Genes CYP76M7 and CYP76M8 Induces Cell Death and Enhances Bacterial Blight Resistance in Indica Rice ‘9311’[J]. International Journal of Molecular Sciences, 2022, 23(13): 7234.(IF 6.208)

[7] Guo F, Yu W, Fu F, et al. Physiological, transcriptome and metabolome analyses provide molecular insights to seasonal development in Ginkgo biloba xylem[J]. Industrial Crops and Products, 2024, 208: 117930.(IF 5.9)

[8] Zhang M, Zhou Y, Yang X, et al. Preparation of esterified biomass waste hydrogels and their removal of Pb2+, Cu2+ and Cd2+ from aqueous solution[J]. Environmental Science and Pollution Research, 2023, 30(19): 56580-56593.(IF 5.8)

[9] Huang Y, Qian C, Lin J, et al. CcNAC1 by Transcriptome Analysis Is Involved in Sudan Grass Secondary Cell Wall Formation as a Positive Regulator[J]. International Journal of Molecular Sciences, 2023, 24(7): 6149.(IF 5.6)

[10] Huang Y, Li Y, Zou K, et al. The Resistance of Maize to Ustilago maydis Infection Is Correlated with the Degree of Methyl Esterification of Pectin in the Cell Wall[J]. International Journal of Molecular Sciences, 2023, 24(19): 14737.(IF 5.6)

[11] Sun W, Chen Y, Zeng J, et al. The Tartary buckwheat bHLH gene ALCATRAZ contributes to silique dehiscence in Arabidopsis thaliana[J]. Plant Science, 2023, 333: 111733.(IF 5.2)

[12] He Y, Gao Y, Hong H, et al. Megalurothrips usitatus Directly Causes the Black-Heads and Black-Tail Symptoms of Cowpea along with the Production of Insect-Resistance Flavonoids[J]. Plants, 2023, 12(22): 3865.(IF 4.5)

[13] Luo X, Wu Z, Fu L, et al. Responses of the lodging resistance of indica rice cultivars to temperature and solar radiation under field conditions[J]. Agronomy, 2022, 12(11): 2603.(IF 3.949)

[14] Wang Y, Zhou E, Yao M, et al. PEG-6000 Priming Improves Aged Soybean Seed Vigor via Carbon Metabolism, ROS Scavenging, Hormone Signaling, and Lignin Synthesis Regulation[J]. Agronomy, 2023, 13(12): 3021.(IF 3.7)

[15] Li Q Y, Wang S, Wu G Y, et al. Physiological and biochemical changes in leaf abscission of Cyclocarya paliurus stem segments in vitro[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2023: 1-11.(IF 3)

[16] Yu H, Yuan Y, Wang S, et al. Interspecies evolution and networks investigation of the auxin response protein (AUX/IAA) family reveals the adaptation mechanisms of halophytes crops in nitrogen starvation agroecological environments[J]. Agriculture, 2021, 11(8): 780.(IF 2.925)

[17] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)

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