Glycogen Content Assay Kit
糖原含量检测试剂盒
货号:AKSU004C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Glycogen Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:2 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    浓硫酸(H2SO4,MW=98.078,CAS: 7664-93-9)
Detection Principle
糖原在强酸性条件下脱水生成 5-羟甲基糠醛,进一步与蒽酮反应生成蓝绿色糠醛衍生物,产物在 620 nm 处具有特征吸收峰,通过吸光值变化即可定量检测糖原的含量。
  • 检测方法: 酸水解-蒽酮比色法
  • 检测波长: 620 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=9.3461x+0.0091(R²=0.9996)
  • 标准线性范围: 0.0125-0.20 mg/mL
  • 检测限: 0.002 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

②浓硫酸具有强腐蚀性,操作时需特别注意,沸水浴显色结束后需冷却至室温再打开离心管盖,以防液体飞溅烧伤;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Sun T, Li Z, Li S, et al. Exploring and validating key factors limiting cyanobacteria-based CO2 bioconversion: Case study to maximize myo-inositol biosynthesis[J]. Chemical Engineering Journal, 2023, 452: 139158.(IF 16.744)

[2] Zhuang M, Rao L, Chen Y, et al. Controlled SPION-Exosomes Loaded with Quercetin Preserves Pancreatic Beta Cell Survival and Function in Type 2 Diabetes Mellitus[J]. International Journal of Nanomedicine, 2023: 5733-5748.(IF 8)

[3] Yuan H, Yuan J, You Y, et al. Simultaneous ammonium and sulfate biotransformation driven by aeration: Nitrogen/sulfur metabolism and metagenome-based microbial ecology[J]. Science of The Total Environment, 2021, 794: 148650.(IF 7.963)

[4] Yuan H, Dang Z, Li C, et al. Simultaneous oxygen and nitrate respiration for nitrogen removal driven by aeration: Carbon/nitrogen metabolism and metagenome-based microbial ecology[J]. Journal of Water Process Engineering, 2022, 50: 103196.(IF 7.34)

[5] Xie J, Chen H, Zheng W, et al. miR-275/305 cluster is essential for maintaining energy metabolic homeostasis by the insulin signaling pathway in Bactrocera dorsalis[J]. PLoS Genetics, 2022, 18(10): e1010418.(IF 6.02)

[6] Liu J, Wang X, Zhu Y, et al. Theabrownin from Dark Tea Ameliorates Insulin Resistance via Attenuating Oxidative Stress and Modulating IRS-1/PI3K/Akt Pathway in HepG2 Cells[J]. Nutrients, 2023, 15(18): 3862.(IF 5.9)

[7] Zhou J, Shi Y, Yang C, et al. γ-glutamylcysteine alleviates insulin resistance and hepatic steatosis by regulating adenylate cyclase and IGF-1R/IRS1/PI3K/Akt signaling pathways[J]. The Journal of Nutritional Biochemistry, 2023: 109404.(IF 5.6)

[8] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

[9] Zhang S, Xie J, Luo R, et al. MiR-2b-2-5p regulates lipid metabolism and reproduction by targeting CREB in Bactrocera dorsalis[J]. RNA biology, 2023, 20(1): 164-176.(IF 4.1)

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