Total Protein Extraction Kit
全蛋白提取试剂盒
货号:AKPR018-1
规格: 50T
检测设备:-
可检测样本数:50 Samples
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Product Information
Total Protein Extraction Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:匀浆器、低温离心机、离心管、可调式移液器
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    液氮
Detection Principle
全蛋白提取试剂盒(Total Protein Extraction Kit)用于哺乳动物组织和细胞中提取总蛋白,Lysis Buffer中含有蛋白酶抑制剂(Protease Inhibitors)和磷酸酶抑制剂(Phosphatase Inhibitors),可快速获得总蛋白,提取蛋白适用于免疫印迹实验等基础研究实验。
  • 检测方法: -
  • 检测波长: -
  • 信号响应: -
Notices

①实验过程,所有试剂都要需要预冷或者即融,保证操作过程中的低温环境;

②PSMF具有毒性且在水溶液中会快速降解,应现用现加,注意做好防护措施;

③Lysis Buffer中含有Protease Inhibitors,提取后蛋白不能用于蛋白激酶和磷酸激酶研究;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Lei Y, Yuan Z, Zeng Q, et al. Dynamic N6-methyladenosine RNA methylation landscapes reveal epi-transcriptomic modulation induced by ammonia nitrogen exposure in the Pacific whiteleg shrimp Litopenaeus vannamei[J]. Journal of Hazardous Materials, 2023, 458: 131996.(IF 13.6)

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