BCA Protein Content Assay Kit
BCA法蛋白含量检测试剂盒
货号:AKPR017
规格: 120-600T
检测设备:可见分光光度计/酶标仪
可检测样本数:100-600 Samples
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Product Information
BCA Protein Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:可见分光光度计(1 mL玻璃比色皿)/酶标仪(96孔板)
  • 预计测定时间:3 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
BCA(Bicinchonininc Acid)蛋白含量检测法是用于总蛋白质定量的常用方法,BCA与二价铜离子混合即为BCA工作液,蛋白在碱性条件下能够将Cu2+还原为Cu+,Cu+与BCA试剂可形成紫色络合物,产物在562 nm处具有特征吸收峰,通过吸光值变化即可定量检测样品的蛋白浓度。
  • 检测方法: BCA
  • 检测波长: 562 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: BSA
  • 参考标准: y=1.8946x+0.1219 (R2=0.9996)
  • 标准线性范围: 0.1-0.5 mg/mL
  • 检测限: 0.05 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①Copper Solution与 PBS Diluent可置于2-8℃长期保存,若发现污染浑浊则应丢弃;BCA Solution在低温条件下出现结晶沉淀时,可37℃温育使其完全溶解,不影响使用;

②为了您的安全和健康,请穿实验服并戴一次性手套操作;

③BCA法测定蛋白含量不受绝大部分样品中化学物质的影响,样品中5% SDS、5% Triton X-100、5% Tween20、60、80等常用浓度去垢剂不影响检测结果,但EDTA和EGTA等螯合剂、DTT和巯基乙醇等还原剂和脂类会影响检测结果,需确保EDTA低于10 mM、无EGTA、二硫苏糖醇低于1 mM、巯基乙醇低于0.01%;若样品含有较多干扰物质且本身背景值较高,建议使用Bradford法蛋白含量检测试剂盒(Cat AKPR015)。

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhang P, Zhou C, Ren X, et al. Inhibiting the compensatory elevation of xCT collaborates with disulfiram/copper-induced GSH consumption for cascade ferroptosis and cuproptosis[J]. Redox Biology, 2023: 103007.(IF 11.4)

[2] Yi Y, Li W, Liu K, et al. Licorice-saponin A3 is a broad-spectrum inhibitor for COVID-19 by targeting viral spike and anti-inflammation[J]. Journal of Pharmaceutical Analysis, 2023.(IF 8.8)

[3] Zhou C, Wang L, Hu W, et al. CDC25C is a prognostic biomarker and correlated with mitochondrial homeostasis in pancreatic adenocarcinoma[J]. Bioengineered, 2022, 13(5): 13089-13107.(IF 6.832)

[4] Xu J, Zhao N, Meng X, et al. Contribution of amino acids to Alicyclobacillus acidoterrestris DSM 3922T resistance towards acid stress[J]. Food Microbiology, 2023, 113: 104273.(IF 5.3)

[5] Wang L, Wang H, Yang C, et al. Investigating CENPW as a Novel Biomarker Correlated With the Development and Poor Prognosis of Breast Carcinoma[J]. Frontiers in Genetics, 2022, 13: 900111.(IF 4.772)

[6] Du P, Song J, Qiu H, et al. Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264. 7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation[J]. Molecules, 2021, 26(9): 2745.(IF 4.412)

[7] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

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