Glutathione Reductase (GR) Activity Assay Kit
谷胱甘肽还原酶活性检测试剂盒
货号:AKPR010M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Glutathione Reductase (GR) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔UV板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
GR能催化NADPH还原GSSG生成GSH,同时NADPH脱氢生成NADP+,NADPH在340 nm处具有特征吸收峰,通过吸光值的变化即可表征谷胱甘肽还原酶的活性。
  • 检测方法: NADPH法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices

①粗酶液制备等过程均需要在冰上进行,建议样品处理后当天完成测定,粗酶液避免反复冻融;

②试剂一中含有一定浓度的蛋白(约0.1 mg/mL),测定样品蛋白浓度时需减去本身的蛋白含量;

③反应过程中应保持37℃(哺乳动物)或25℃(其他物种)恒温,建议在烧杯中装入适量37℃(哺乳动物)或25℃(其他物种)蒸馏水,将此烧杯放入37℃(哺乳动物)或25℃(其他物种)恒温水浴中,在反应过程中把比色皿连同反应液放在此烧杯中;

④准确在10 s和190 s处完成读数,以保证实验结果的准确性;若使用96孔UV板测定,需使用多道移液器且分批进行,以确保组间反应时间一致;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yao X, Zhou M, Ruan J, et al. Physiological and Biochemical Regulation Mechanism of Exogenous Hydrogen Peroxide in Alleviating NaCl Stress Toxicity in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)[J]. International Journal of Molecular Sciences, 2022, 23(18): 10698.(IF 6.208)

[2] Tao D, Wang Y, Liu J, et al. Mechanism of CuSO4 cytotoxicity in goat erythrocytes after high-level in vitro exposure to isotonic media[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111730.(IF 4.872)

[3] Xu J, Kang Z, Zhu K, et al. RBOH1-dependent H2O2 mediates spermine-induced antioxidant enzyme system to enhance tomato seedling tolerance to salinity–alkalinity stress[J]. Plant Physiology and Biochemistry, 2021, 164: 237-246.(IF 4.27)

[4] Zhong Z, Li H, Li Z, et al. Inhibiting thioredoxin glutathione reductase is a promising approach to controlling Cryptocaryon irritans infection in fish[J]. Veterinary Parasitology, 2023: 109972.(IF 2.6)

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