Glutathione S-Transferase (GST) Activity Assay Kit
谷胱甘肽S-转移酶活性检测试剂盒
货号:AKPR013U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
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Product Information
Glutathione S-Transferase (GST) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
谷胱甘肽S-转移酶催化还原型谷胱甘肽(GSH)与CDNB结合生成GS-DNB,产物在340 nm处具有特征吸收峰,通过吸光值变化即可表征谷胱甘肽S-转移酶的活性。
  • 检测方法: CDNB法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices

①样品处理等过程均需要在冰上进行以免失活,粗酶液制备完成后须当天完成测定;

②测定细胞中GST活性时,细胞数目建议在300-500万之间,细胞中GST的提取时可加试剂一后研磨或超声波处理,不能用细胞裂解液处理细胞;

③若测定吸光度大于1,建议将粗酶液使用蒸馏水稀释后再进行测定,计算时相应修改;

④反应过程中应保持37℃(哺乳动物)或25℃(其他物种)恒温,建议在烧杯中装入适量37℃(哺乳动物)或25℃(其他物种)蒸馏水,将此烧杯放入37℃(哺乳动物)或25℃(其他物种)恒温水浴中,在反应过程中把比色皿连同反应液放在此烧杯中;

⑤准确在10 s和310 s处完成读数,以保证实验结果的准确性;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Liu T, Wang J, Chen L, et al. ScAREB4 promotes potato constitutive and acclimated freezing tolerance associated with enhancing trehalose synthesis and oxidative stress tolerance[J]. Plant, Cell & Environment, 2023, 46(12): 3839-3857.(IF 7.3)

[2] Ma W, Sun Z, Zhang X, et al. Alleviation of tris (2-chloroethyl) phosphate toxicity on the marine rotifer Brachionus plicatilis by polystyrene microplastics: features and molecular evidence[J]. International Journal of Molecular Sciences, 2022, 23(9): 4934.(IF 6.208)

[3] Zhou T, Wu J, Liu Y, et al. Seawater Accelerated the Aging of Polystyrene and Enhanced Its Toxic Effects on Caenorhabditis elegans[J]. International Journal of Molecular Sciences, 2023, 24(24): 17219.(IF 5.6)

[4] Zhang Y, Ni C, Dong Y, et al. The Role of the Ascorbic Acid–Glutathione Cycle in Young Wheat Ears’ Response to Spring Freezing Stress[J]. Plants, 2023, 12(24): 4170.(IF 4.5)

[5] Wang Y, Zhou E, Yao M, et al. PEG-6000 Priming Improves Aged Soybean Seed Vigor via Carbon Metabolism, ROS Scavenging, Hormone Signaling, and Lignin Synthesis Regulation[J]. Agronomy, 2023, 13(12): 3021.(IF 3.7)

[6] Zhang S, Zhang D, Jia Y, et al. Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta[J]. Journal of Applied Entomology, 2023, 147(4): 249-260.(IF 1.9)

[7] Ren H, Zhang H, Tan Y, et al. Sublethal effects of chlorantraniliprole on biological characteristics, detoxifying enzyme activity and gene expression profile in the Allium mongolicum Regel leaf beetle Galeruca daurica (Coleoptera: Chrysomelidae)[J]. Journal of Applied Entomology, 2023.(IF 1.9)

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