Pepsin Activity Assay Kit
胃蛋白酶活性检测试剂盒
货号:AKPR006U
规格: 60T/25S
检测设备:紫外分光光度计
可检测样本数:25 Samples
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Product Information
Pepsin Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:3 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
胃蛋白酶能够催化血红蛋白水解生成酪氨酸,产物在275 nm处具有特征吸收峰,通过吸光值变化即可表征胃蛋白酶的活性。
  • 检测方法: 酪氨酸比色法
  • 检测波长: 275 nm
  • 信号响应: 递增型
Notices
①若A测定大于1.2,建议将粗酶液适当稀释后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Hou G, Zhao X, Zhao T, et al. The adsorption of PAHs on microplastics and desorption in the simulated human digestive system[J]. Chemical Engineering Journal, 2023, 473: 145157.(IF 15.1)

[2] Li T J, Wen B Y, Ma X H, et al. Rapid and simple analysis of the human pepsin secondary structure using a portable Raman spectrometer[J]. Analytical Chemistry, 2021, 94(2): 1318-1324.(IF 6.986)

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