Trypsase (TRY) Activity Assay Kit
胰蛋白酶活性检测试剂盒
货号:AKPR005M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Trypsase (TRY) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔UV板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
胰蛋白酶能够催化N-苯甲酰-L-精氨酸乙酯(N-benzoyl-L-arginine-ethylester,BAEE)中酯键水解,生成N-苯甲酰-L-精氨酸(N-benzoyl-L-arginine,BA),产物在253 nm处具有特征吸收峰,通过吸光值的变化即可表征胰蛋白酶的活性。
  • 检测方法: BAEE法
  • 检测波长: 253 nm
  • 信号响应: 递增型
Notices

①建议调整样本量或稀释程度将吸光值变化控制在0.01-0.15之间,可提高检测精准度;

②若∆A测定为负值,建议将试剂一适当稀释(2-5倍)后再进行实验;

③准确在0 s和60 s处完成读数,以保证实验结果的准确性;若使用96孔板应使用多道移液器且分批进行检测,以确保组间反应时间一致;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wang B, Wang Y, He W, et al. StMLP1, as a Kunitz trypsin inhibitor, enhances potato resistance and specifically expresses in vascular bundles during Ralstonia solanacearum infection[J]. The Plant Journal, 2023.(IF 7.2)

[2] Hou G, Zhao X, Zhao T, et al. The adsorption of PAHs on microplastics and desorption in the simulated human digestive system[J]. Chemical Engineering Journal, 2023, 473: 145157.(IF 15.1)

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