DPPH Free Radical Scavenging Ability Assay Kit
DPPH自由基清除能力检测试剂盒
货号:AKAO020M
规格: 240T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
DPPH Free Radical Scavenging Ability Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:6 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
DPPH自由基具有单电子,其有机溶液呈紫色,在515 nm处具有特征吸收峰,当有抗氧化剂存在时,DPPH自由基接受一个电子或氢原子,形成稳定的DPPH-H化合物,使溶液从紫色变为黄色,变色程度与其自由基清除活性呈定量关系,通过吸光度的变化即可表征DPPH自由基清除能力,并提供Trolox作为阳性对照量化抗氧化物质的清除能力。
  • 检测方法: DPPH法
  • 检测波长: 515 nm
  • 信号响应: 递减型
Refreence Information
  • 标准物质: Trolox
  • 参考标准: y=60.054x-0.7976(R2=0.9997)
  • 标准线性范围: 0.1-1.6 mmol/L
  • 检测限: -
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①样品提取过程建议在冰上完成操作,且提取后应当天完成测定;

②若待测样本DPPH自由基清除率(DS%)大于90%,建议将待测样本使用提取液稀释后再进行测定;若待测样本DPPH自由基清除率(DS%)小于5%,建议适当增加烘干样本质量或液体样本体积重新提取后再进行测定,计算时相应修改;

③不同样本DPPH自由基清除能力可能相差较大,若需要比较不同样本的DPPH自由基清除能力,建议对于同一批植物样本加入等量的样本,液体样本加入相同体积,提取物或者药物配制为相同浓度;将样本根据预实验结果进行适当调整,比较同样浓度(相同稀释倍数)的清除率大小;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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