Peroxidase (POD) Activity Assay Kit
过氧化物酶活性检测试剂盒
货号:AKAO005C
规格: 60T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Peroxidase (POD) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
过氧化物酶可催化H2O2氧化愈创木酚生成茶褐色4-邻甲氧基苯酚,产物在470 nm处具有特征吸收峰,通过吸光值变化即可表征过氧化物酶的活性。
  • 检测方法: 愈创木酚法
  • 检测波长: 470 nm
  • 信号响应: 递增型
Notices

①若测定样本较多,可将试剂一、试剂二和试剂三按比例配成检测工作液(现用现配),37℃(哺乳动物)或25℃(其它物种)预热10 min以上,测定时加入50 μL粗酶液和950 μL检测工作液;

②准确在相应时间点完成吸光值测定,以确保实验结果的准确性和重复性;

③若ΔA小于0.005,可适当延长反应时间(3-5 min)后再进行测定;若ΔA大于0.5,建议将粗酶液使用提取液稀释后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhao Y, Li Y, Jin Y, et al. The inhibitory effects of Ulva prolifera extracts on early growth of Spartina alterniflora and the underlying mechanisms[J]. Journal of Environmental Management, 2022, 319: 115639.(IF 8.91)

[2] Zhu K, Yan D, Wang Y, et al. Chlorophyll retention reduces storability and pathogen defense in a novel citrus brown flavedo mutant[J]. Postharvest Biology and Technology, 2022, 192: 112006.(IF 6.751)

[3] Yao X, Zhou M, Ruan J, et al. Physiological and Biochemical Regulation Mechanism of Exogenous Hydrogen Peroxide in Alleviating NaCl Stress Toxicity in Tartary Buckwheat (Fagopyrum tataricum (L.) Gaertn)[J]. International Journal of Molecular Sciences, 2022, 23(18): 10698.(IF 6.208)

[4] Li J, Cai B, Chang S, et al. Mechanisms associated with the synergistic induction of resistance to tobacco black shank in tobacco by arbuscular mycorrhizal fungi and β-aminobutyric acid[J]. Frontiers in Plant Science, 2023, 14.(IF 5.6)

[5] Li Z, Li X, He F. Drip Irrigation Depth Alters Root Morphology and Architecture and Cold Resistance of Alfalfa[J]. Agronomy, 2022, 12(9): 2192.(IF 3.949)

[6] Yao X, Zhou M, Ruan J, et al. Pretreatment with H2O2 alleviates the negative impacts of NaCl stress on seed germination of Tartary buckwheat (Fagopyrum tataricum)[J]. Plants, 2021, 10(9): 1784.(IF 3.935)

[7] Zhang L, Zhang R, Ye X, et al. Overexpressing VvWRKY18 from grapevine reduces the drought tolerance in Arabidopsis by increasing leaf stomatal density[J]. Journal of Plant Physiology, 2022, 275: 153741.(IF 3.686)

[8] Li Q Y, Wang S, Wu G Y, et al. Physiological and biochemical changes in leaf abscission of Cyclocarya paliurus stem segments in vitro[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2023: 1-11.(IF 3)

[9] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)

[10] Liang Y E, Zhang H, Zhu J, et al. Transcriptomic Analysis Reveals the Involvement of Flavonoids Synthesis Genes and Transcription Factors in Dracaena cambodiana Response to Ultraviolet-B Radiation[J]. Forests, 2023, 14(5): 979.(IF 2.9)

[11] Jing S, Begum S, Yu L, et al. StJAZ1-like mediated root architecture plays critical roles in drought susceptibility in potato[J]. Environmental and Experimental Botany, 2022, 202: 105008.(IF 6.028)

[12] Song X, Hou X, Zeng Y, et al. Genome-wide identification and comprehensive analysis of WRKY transcription factor family in safflower during drought stress[J]. Scientific Reports, 2023, 13(1): 16955.(IF 4.6)

[13] Li L, Wu S, Wang S, et al. Molecular Mechanism of Exogenous Selenium Affecting the Nutritional Quality, Species and Content of Organic Selenium in Mustard[J]. Agronomy, 2023, 13(5): 1425.(IF 3.7)

[14] Liu Y, Zhou J, Chen Y, et al. GmSTK12 Participates in Salt Stress Resistance in Soybean[J]. Agronomy, 2023, 13(2): 613.(IF 3.7)

[15] Wang Y, Zhou E, Yao M, et al. PEG-6000 Priming Improves Aged Soybean Seed Vigor via Carbon Metabolism, ROS Scavenging, Hormone Signaling, and Lignin Synthesis Regulation[J]. Agronomy, 2023, 13(12): 3021.(IF 3.7)

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