Protein Carbonyl Content Assay Kit
蛋白质羰基含量检测试剂盒
货号:AKAO007M
规格: 110T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Protein Carbonyl Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔UV板
  • 预计测定时间:7 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    乙酸乙酯(C4H8O2,MW=88.10,CAS: 141-78-6);
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
蛋白质羰基能够与2,4-二硝基苯肼反应生成红色2,4-二硝基苯腙,产物在370 nm处具有特征吸收峰,通过吸光值变化即可定量检测蛋白质羰基的含量。
  • 检测方法: 2,4-二硝基苯肼法
  • 检测波长: 370 nm
  • 信号响应: 递增型
Notices

①提取液B应根据使用量现用现配,配置后置于4℃保存,若变为黑色则停止使用;

②试剂一见光易分解,反应过程需在避光条件下进行;

③试剂四为沉淀洗涤液,需重复加入三次对沉淀进行洗涤;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] He F, Shi H, Guo S, et al. Molecular mechanisms of nano-sized polystyrene plastics induced cytotoxicity and immunotoxicity in Eisenia fetida[J]. Journal of Hazardous Materials, 2023: 133032.(IF 13.6)

[2] Shi J, Xiong Z, Wang K, et al. HIF2α promotes tumour growth in clear cell renal cell carcinoma by increasing the expression of NUDT1 to reduce oxidative stress[J]. Clinical and Translational Medicine, 2021, 11(11): e592.(IF 11.492)

[3] Yang H, Li Q, Xu Z, et al. Preparation of three-layer flaxseed gum/chitosan/flaxseed gum composite coatings with sustained-release properties and their excellent protective effect on myofibril protein of rainbow trout[J]. International Journal of Biological Macromolecules, 2022, 194: 510-520.(IF 6.953)

[4] Gao X, Wang Z, Xiong L, et al. The bs-YHEDA peptide protects the brains of senile mice and thus recovers intelligence by reducing iron and free radicals[J]. Free Radical Biology and Medicine, 2022, 190: 216-225.(IF 8.101)

[5] He F, Hu S, Liu R, et al. Decoding the biological toxicity of phenanthrene on intestinal cells of Eisenia fetida: Effects, toxicity pathways and corresponding mechanisms[J]. Science of The Total Environment, 2023, 904: 166903.(IF 9.8)

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