Catalase (CAT) Activity Assay Kit
过氧化氢酶活性检测试剂盒(钼酸铵比色法)
货号:AKAO003-2C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Catalase (CAT) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
H2O2能够氧化MoO42-生成MoO52-,MoO52-接受氢氧根的电子成键,分子间立即脱水缩合,分子内形成氢键,得到稳定的黄色复合物(H2MoO4·XH2O)n,产物在405 nm处具有特征吸收峰,通过吸光值变化即可表征过氧化氢酶的活性。
  • 检测方法: 钼酸铵比色法
  • 检测波长: 405 nm
  • 信号响应: 递减型
Refreence Information
  • 标准物质: Hydrogen Peroxide
  • 参考标准: y=0.0101x+0.0017 (R2=0.9999)
  • 标准线性范围: 10-100 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准吸光值线性范围:高于最高值建议适当增加样本量重新提取后再进行测定,低于最低值建议将粗酶液使用提取液适当稀释后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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