①若测定样本较多,可将试剂一、试剂二和试剂三按比例配成检测工作液(现用现配),37℃(哺乳动物)或25℃(其它物种)预热10 min以上,测定时加入10 μL粗酶液和190 μL检测工作液;
②若使用96孔板进行检测,应使用多道移液器且分批进行检测,以确保组间反应时间一致;
③若ΔA小于0.005,可适当延长反应时间(3-5 min)后再进行测定;若ΔA大于0.5,建议将粗酶液使用提取液稀释后再进行测定,计算时相应修改;
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