Total Antioxidant Capacity (T-AOC) Assay Kit
总抗氧化能力检测试剂盒
货号:AKAO012M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
In Stock
BOXBIO提供专业的指标检测服务,如需检测服务,请点击右侧按钮
检测服务
Product Information
Total Antioxidant Capacity (T-AOC) Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    浓硫酸(H2SO4,MW=98.078,CAS: 7664-93-9)
Detection Principle
本产品采用FRAP(Ferric Reducing Ability of Plasma)法测定总抗氧化能力,其原理是酸性条件下抗氧化物可以将Fe3+-TPTZ还原为蓝色的络合物Fe2+-TPTZ,产物在593 nm处具有特征吸收峰,通过吸光值的变化可以反映待测样品的还原能力,即样品的总抗氧化能力。
  • 检测方法: FRAP法
  • 检测波长: 593 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: FeSO4
  • 参考标准: y=9.9184x-0.0008 (R2=0.9999)
  • 标准线性范围: 0.003125-0.1 μmol/mL
  • 检测限: 0.0006 μmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂二具有刺激性应避免接触,注意采取适当的防护措施,请穿实验服并戴乳胶手套操作;

②尽量避免使用在酸性条件下呈蓝色或接近蓝色的样本,否则对本试剂盒的检测结果产生干扰;

③样品中不宜添加Tween和NP-40等去垢剂和DTT、巯基乙醇等影响氧化还原反应的还原剂;

④若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Lin X, Chang M, Cao M, et al. Trimetallic-doped carbon nitride achieves chondroitin sulfate degradation via a free radical degradation strategy[J]. Carbohydrate Polymers, 2024: 122616. (IF 10.7)

[2] Sun J, Yan B, Chen H, et al. Insight into the mechanisms of combined toxicity of cadmium and flotation agents in luminescent bacteria: Role of micro/nano particles[J]. Science of The Total Environment, 2024: 173588. (IF 9.8)

[3] Zhou D, Hu Y, Zhou T, et al. Deep eutectic solvent extraction of polysaccharides from Gastrodiae elata with ultrasonic and enzymes assistance: Process optimization, structure, and antioxidant activity[J]. Ultrasonics Sonochemistry, 2025: 107383. (IF 8.7)

[4] Cai Z, Zhou S, Zhang T, et al. Synergistic enhancement of bio-yogurt properties by Lactiplantibacillus plantarum NUC08 and mulberry fruit extract[J]. Food Chemistry, 2024: 142447. (IF 8.5)

[5] Zhang Y, Liu Y, Liu X, et al. Exercise and metformin intervention prevents lipotoxicity-induced hepatocyte apoptosis by alleviating oxidative and ER stress and activating the AMPK/Nrf2/HO-1 signaling pathway in db/db mice[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022.(IF 7.31)

[6] Wang Z, Jiang Q, Li P, et al. The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation[J]. Antioxidants, 2023, 12(3): 547.(IF 7)

[7] Li K, Xu R, Cao J, et al. Nordihydroguaiaretic acid (NDGA) maintains the postharvest quality of fresh daylily flower buds (Hemerocallis citrina) by enhancing antioxidant metabolism and regulating phytohormone equilibrium[J]. Postharvest Biology and Technology, 2023, 200: 112326.(IF 7)

[8] Chen Y, Xu Y, Li D, et al. Exogenous azacytidine alleviates peel browning of postharvest bananas (Musa acuminata) showing an important role of DNA methylation[J]. Postharvest Biology and Technology, 2023, 206: 112554.(IF 7)

[9] Xiang X, Wang H, Zhou W, et al. Glutathione Protects against Paraquat-Induced Oxidative Stress by Regulating Intestinal Barrier, Antioxidant Capacity, and CAR Signaling Pathway in Weaned Piglets[J]. Nutrients, 2023, 15(1): 198.(IF 6.706)

[10] Chen J, Li Y, Li F, et al. Physiological, metabolome, and transcriptome analysis revealed the effect of ethyl 3-amino-3-thioxopropanoate on the browning of fresh-cut banana during storage[J]. Postharvest Biology and Technology, 2024, 218: 113176. (IF 6.4)

[11] Xing S, Tian Q, Zheng Y, et al. Sulfur dioxide enhances the resistance of postharvest grape berries to gray mold through hydrogen peroxide signaling[J]. Postharvest Biology and Technology, 2025, 221: 113325. (IF 6.4)

[12] Tang W, Xiang X, Wang H, et al. Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets[J]. Animal Nutrition, 2023.(IF 6.3)

[13] Yue Z, Guo Y, Zhang Y, et al. Endophytic Pantoea sp. EEL5 isolated from Elytrigia elongata improves wheat resistance to combined salinity-cadmium stress by affecting host gene expression and altering the rhizosphere microenvironment[J]. Industrial Crops and Products, 2025, 234: 121585. (IF 6.2)

[14] Wang M, Jiang Y, Wang S, et al. Yak milk protects against alcohol-induced liver injury in rats[J]. Food & Function, 2023, 14(21): 9857-9871.(IF 6.1)

[15] Zeng C, Li R, Liao Y, et al. Effect of vacuum microwave drying pretreatment on the production, characteristics, and quality of Jujube powder[J]. LWT, 2025: 117674. (IF 6.0)

[16] Pan T, Li M, Guo J, et al. Bacillus safensis from Sauerkraut Alleviates Acute Lung Injury Induced by Methicillin-Resistant Staphylococcus aureus through the Regulation of M2 Macrophage Polarization via Its Metabolite Esculin[J]. Journal of Agricultural and Food Chemistry, 2024. (IF 5.7)

[17] Tang J, Zhu Z, He H, et al. Bacterioferritin: a key iron storage modulator that affects strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona[J]. Microbial Cell Factories, 2021, 20(1): 1-16.(IF 5.328)

[18] Yang Z, Wang F, Yin Y, et al. Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets[J]. Animal Nutrition, 2023, 13: 9-18.(IF 5.285)

[19] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

[20] Lin Z, Li Y, Wang M, et al. Protective effects of yeast extract against alcohol-induced liver injury in rats[J]. Frontiers in Microbiology, 2023, 14.(IF 5.2)

[21] Qian Y, Guo Y, Li Y, et al. The antihypertensive effect of Alizarin is achieved by activating VEGFR2/eNOS pathway, attenuating oxidative stress-induced mitochondrial damage and premature senescence[J]. Life Sciences, 2024: 122862. (IF 5.2)

[22] Ye Z, Shang C, Wang Y, et al. Ultrathin PtTeNi nanosheets as a peroxidase mimic for colorimetric detection of ascorbic acid[J]. Microchemical Journal, 2024: 111919. (IF 4.9)

[23] Song T, Li R, Zhang M, et al. Construction of wheat gluten amyloid fiber/lutein complex delivery system: Formation mechanism, in vitro bioaccessibility, and photothermal stability[J]. Food Bioscience, 2024: 105800. (IF 4.8)

[24] Zhang G Q, Liu W, Shi Y H, et al. Intrinsic peroxidase activity of carbon dots derived from expired drugs and its application in automatic detection of total antioxidant capacity[J]. Microchemical Journal, 2023: 109720.(IF 4.8)

[25] Wan B, Tian T, Xiong Y, et al. Isolation and Evaluation of Rhizopus arrhizus Strains from Traditional Rice Wine Starters (Jiuqu): Enzyme Activities, Antioxidant Capacity, and Flavour Compounds[J]. Foods, 2025, 14(2): 312. (IF 4.7)

[26] Tang M, Zhao J, Wu Y, et al. Improving Gut Functions and Egg Nutrition with Stevia Residue in Laying Hens: METABOLISM AND NUTRITION[J]. Poultry Science, 2023: 103324.(IF 4.4)

[27] Li C, Cheng Y, Zong T, et al. Alleviative Effect of Vaginal Lactobacilli with Probiotic Potential from Healthy Chinese Women on Bacterial Vaginosis Caused by Gardnerella vaginalis in Mice[J]. Probiotics and Antimicrobial Proteins, 2025: 1-17. (IF 4.4)

[28] Shi J, Zhou Y, Yang S, et al. The key metabolic pathway of roots and leaves responses in Arachis hypogaea under Al toxicity stress[J]. BMC Plant Biology, 2025, 25(1): 439. (IF 4.3)

[29] Liao L, Zhang W, Liu Y, et al. Optimization of fermentation process for Sihuang compound with Bacillus subtilis and its efficacy against Escherichia coli infections and growth performance in broilers[J]. Poultry Science, 2025: 105588. (IF 4.2)

[30] Wang W, Wang Y, Huang P, et al. Mosla Chinensis Extract Enhances Growth Performance, Antioxidant Capacity, and Intestinal Health in Broilers by Modulating Gut Microbiota[J]. Microorganisms, 2024, 12(12): 2647. (IF 4.1)

[31] Yi X, Zhang S, Meng D, et al. Optimization of Rosa roxburghii Tratt pomace fermentation process and the effects of mono-and mixed culture fermentation on its chemical composition[J]. Frontiers in Nutrition, 2024, 11: 1494678. (IF 4.0)

[32] Zhang H, Xiang X, Wang C, et al. Different effects of acute and chronic oxidative stress on the intestinal flora and gut-liver axis in weaned piglets[J]. Frontiers in Microbiology, 2024, 15: 1414486. (IF 4.0)

[33] Zhao Y, Wang H, Mei Y, et al. Profiling Metabolites Distribution among Various Leaf Layers of Chinese Cabbage[J]. Horticulturae, 2024, 10(9): 988. (IF 3.1)

[34] Yang Z, Sun G, Tao J, et al. Dietary Tea Polyphenols Improve Growth Performance and Intestinal Microbiota Under Chronic Crowding Stress in Hybrid Crucian Carp[J]. Animals, 2025, 15(13): 1983. (IF 2.7)

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司