

①不同样本的多酚氧化酶最佳反应温度略有差别,反应温度可在25-37℃之间进行调整;
②若A测定大于1.5或ΔA大于1.0,建议将粗酶液适当稀释后再进行测定;若ΔA小于0.02,建议适当延长酶促反应时间或适当增加样本量后再进行测定,计算时相应修改;
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