

①若∆A大于0.3,建议将粗酶液使用提取液适当稀释后再进行测定;若∆A小于0.05,建议适当增加酶促反应时间或增加样本量后再进行测定,计算时相应修改;
②试剂一配制后易氧化,应现用现配,配制后尽快用完;
③准确在10 s和190 s时完成吸光值测定,以确保实验结果的准确性和重复性;若使用96孔板进行测定,应使用多道移液器且分批进行测定,以确保各组间反应时间的一致;
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