

①测定过程中样本应保持冰上放置,以免变性和失活;
②准确在20 s和320 s处完成读数,以保证实验结果的准确性;若使用96孔UV板进行检测,应使用多道移液器且分批进行测定,以确保组间反应时间一致;
③若∆A大于0.5,建议将样本适当稀释后再进行测定,计算时相应修改;
④提取液中含有约1 mg/mL的蛋白,测定样本蛋白浓度时需要减去提取液的蛋白含量;
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