

①试剂二配制后有效期较短,为便于试验安排,附赠一瓶试剂二作为备用,每瓶均可完成至少25个样本的检测;
②测定过程中粗酶液和试剂二应置于冰上放置,建议粗酶液提取完成后当天完成活性检测;
③若A测定>1.0,建议将粗酶液使用提取液适当稀释后再进行测定;若A测定<0.02,建议适当延长酶促反应时间(37℃准确反应5 min,可以延长至30 min以上)或制备更高浓度的样本后再进行测定,计算时相应修改;
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