①反应过程中加入试剂三后必须立即充分混匀,否则会导致显色不完全;
②粗酶液制备完成后需置于冰上放置,且当天完成活性检测;
③若测定吸光值超出标准吸光值线性范围:高于最高值建议将粗酶液适当稀释后再进行测定,低于最低值建议适当增加样本量或延长反应时间后再进行测定,计算时相应修改;
④试剂三颜色应为黄色,变为蓝绿色则已失效应停止使用;
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