Acetyl Coenzyme A Content Assay Kit
乙酰辅酶A含量检测试剂盒
货号:AKFA019U-2
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
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Product Information
Acetyl Coenzyme A Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
苹果酸脱氢酶能够催化苹果酸和NAD生成草酰乙酸和NADH,柠檬酸合酶可催化乙酰辅酶A和草酰乙酸生成柠檬酸和辅酶A,通过苹果酸脱氢酶和柠檬酸合酶的偶联反应体系,乙酰辅酶A含量和NADH的生成速率成正比,NADH在340 nm处具有特征吸收峰,通过吸光值的变化速率即可定量检测乙酰辅酶A的含量。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Acetyl Coenzyme A
  • 参考标准: To Be Updated
  • 标准线性范围: To Be Updated
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Xiang F, Liu W C, Liu X, et al. Direct balancing of lipid mobilization and ROS production by the epoxidation of fatty acid catalyzed by a cytochrome P450 protein during seed germination[J]. The New Phytologist, 2022.(IF 10.323)

[2] Lin T, Yang W, Luo W, et al. Disturbance of Fatty Acid Metabolism Promoted Vascular Endothelial Cell Senescence via Acetyl-CoA-Induced Protein Acetylation Modification[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022.(IF 7.31)

[3] Tian W, Liu Y, Zhang W, et al. CircDOCK7 facilitates the proliferation and adipogenic differentiation of chicken abdominal preadipocytes through the gga-miR-301b-3p/ACSL1 axis[J]. Journal of Animal Science and Biotechnology, 2023, 14(1): 91.(IF 7)

[4] Shen N, Li S, Li S, et al. Reduced acetic acid formation using NaHSO3 as a steering agent by Actinobacillus succinogenes GXAS137[J]. Journal of bioscience and bioengineering, 2023, 135(3): 203-209.(IF 2.8)

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