Total Cholesterol (TC) Content Assay Kit
总胆固醇含量检测试剂盒
货号:AKFA002M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Total Cholesterol (TC) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    异丙醇(C3H8O,MW=60.06,CAS: 67-63-0)
Detection Principle
胆固醇酯酶可催化胆固醇酯水解生成游离胆固醇(FC)和游离脂肪酸(FFA),胆固醇氧化酶可催化游离胆固醇氧化生成∆4-胆甾烯酮和H2O2,过氧化物酶催化H2O2氧化4-氨基安替比林和酚生成红色醌类化合物,产物在500 nm处具有特征吸收峰,通过吸光值变化即可定量检测总胆固醇的含量。
  • 检测方法: 胆固醇氧化酶法
  • 检测波长: 500 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Cholesterol
  • 参考标准: y=0.3357x-0.0046 (R2=0.9993)
  • 标准线性范围: 0.125-3.0 μmol/mL
  • 检测限: 0.12 μmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若∆A测定超出标准线性吸光值范围:高于最高值将待测样本使用提取液适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Xu J, Khan H, Yang L. Hydrogel paper-based analytical devices: separation-free in situ assay of small-molecule targets in whole blood[J]. Analytical Chemistry, 2021, 93(44): 14755-14763.(IF 6.986)

[2] Yu J, Sun H, Yang Y, et al. Sesamolin alleviates nonalcoholic fatty liver disease through modulating gut microbiota and metabolites in high-fat and high-fructose diet-fed mice[J]. International Journal of Molecular Sciences, 2022, 23(22): 13853.(IF 6.208)

[3] Song J, Qiu H, Du P, et al. Polyphenols extracted from Shanxi‐aged vinegar exert hypolipidemic effects on OA‐induced HepG2 cells via the PPARα‐LXRα‐ABCA1 pathway[J]. Journal of food biochemistry, 2022, 46(2): e14029.(IF 3.654)

[4] He H, Qi R, Cui J, et al. Lipid characteristics of lung tissue in silicosis rat model were studied based on lipid metabolomics[J]. Toxicology Letters, 2024, 391: 111-119.(IF 3.5)

[5] Wang X, Huangfu W, Zhao F. Correlation of ChREBP Gene Methylation with Pathological Characteristics of Type 2 Diabetes Mellitus[J]. Applied Biochemistry and Biotechnology, 2023: 1-12.(IF 3)

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