Carboxylesterase (CarE) Activity Assay Kit
羧酸酯酶活性检测试剂盒
货号:AKFA004C
规格: 50T/48S
检测设备:可见分光光度计
可检测样本数:48 Samples
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Product Information
Carboxylesterase (CarE) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
羧酸酯酶能够催化乙酸-1-萘酯生成萘酯,萘酯与固蓝显色可生成偶氮化合物,产物在450 nm处具有特征吸收峰,通过吸光值的变化即可表征羧酸酯酶的活性。
  • 检测方法: 固蓝显色法
  • 检测波长: 450 nm
  • 信号响应: 递增型
Notices

①动物组织样品一般稀释10倍后进行测定;

②若吸光值大于1时,建议将样品稀释后测量,计算公式注意乘以稀释倍数;

③建议反应时保持37℃的环境,准确在10 s和190 s处完成比色,以保证实验结果的准确性。

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Fan X, Gu C, Cai J, et al. Mechanistic insights into primary biotransformation of diethyl phthalate in earthworm and significant SOD inhibitory effect of esterolytic products[J]. Chemosphere, 2022, 288: 132491.(IF 7.086)

[2] Fan X, Gu C, Jin Z, et al. Major biotransformation of phthalic acid esters in Eisenia fetida: Mechanistic insights and association with catalytic enzymes and intestinal symbionts[J]. Environment International, 2023, 171: 107712.(IF 13.352)

[3] Li J, Jia Y, Zhang D, et al. Molecular identification of carboxylesterase genes and their potential roles in the insecticides susceptibility of Grapholita molesta[J]. Insect Molecular Biology, 2023, 32(3): 305-315.(IF 2.6)

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