

①反应结束后生成的红色产物三甲川可能与样本中大分子物质形成复合物:最后一步沸水浴处理后离心取上清时,正常情况下沉淀不应出现红色,若沉淀中有红色物质出现表明三甲川产物与大分子物质形成了复合物,此时上清中产物浓度是被低估的,会造成结果误差;
Boxbio-丙二醛含量检测试剂盒中有样本中大分子物质专用去除试剂与步骤: 能够有效防止三甲川与大分子物质形成复合物, 避免沉淀出现产物造成误差情况,确保实验结果的准确,建议注意观察最后一步离心沉淀颜色情况,若有异常情况请随时与技术支持取得联系: 400-805-8228(7×24 h);
②植物样本中受蔗糖干扰较大,动物中受葡萄糖干扰较大,本试剂盒有针对蔗糖和葡萄糖的两个公式,若所测样品为油脂类物质,则两个公式均可;
③吸光值正常范围: 532 nm处为产物特征峰,需控制∆A532在0.05-1.0范围之内, 若吸光值未在正常范围内,可参照下述方法进行调整,计算时相应修改计算公式参数即可,建议做好预实验。
· 若∆A532小于0.05: 需制备更高浓度样本后再进行测定, 组织样本待测样本浓度可由10%(w/v)最高提高至20%(w/v), 细菌或细胞类样本待测样本浓度可由500万/mL最高提高至2000万/mL;
· 若∆A532大于1.0: 建议将待测样本使用蒸馏水适当稀释后再进行测定;
④450 nm 和 600 nm 为样本中糖类物质干扰物特征峰, ∆A450 和∆A600 与样本中干扰物含量相关, ∆A450 和∆A600 控制在 1.0 以下即可;
⑤更换波长后在检测前需再次使用蒸馏水对分光光度计进行调零;
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