Malondialdehyde (MDA) Content Assay Kit
丙二醛含量检测试剂盒
货号:AKFA013C
规格: 60T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
In Stock
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Product Information
Malondialdehyde (MDA) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
丙二醛在酸性和高温条件下, 能够与硫代巴比妥酸(Thiobarbituric Acid, TBA)反应生成红色三甲川(3,5,5-三甲基恶唑-2,4-二酮), 产物在 532 nm 处具有特征吸收峰, 通过吸光值变化即可定量检测丙二醛的含量。 需要特殊注意的是测定动植物组织中丙二醛含量时受多种物质的干扰,其中最主要的是可溶性糖, 可溶性糖同样能够与硫代巴比妥酸显色, 最大吸收波长为 532 nm,但 450 nm 处也有吸收, 所以必须同时测定 600 nm、 532 nm、 450 nm 处吸光值, 通过 532 nm、 450 nm 和 600 nm 处吸光值的差值即可准确定量检测丙二醛的含量。
  • 检测方法: TBA法
  • 检测波长: 450 nm & 532 nm & 600 nm
  • 信号响应: 递增型
Notices

①反应结束后生成的红色产物三甲川可能与样本中大分子物质形成复合物:最后一步沸水浴处理后离心取上清时,正常情况下沉淀不应出现红色,若沉淀中有红色物质出现表明三甲川产物与大分子物质形成了复合物,此时上清中产物浓度是被低估的,会造成结果误差;

Boxbio-丙二醛含量检测试剂盒中有样本中大分子物质专用去除试剂与步骤: 能够有效防止三甲川与大分子物质形成复合物, 避免沉淀出现产物造成误差情况,确保实验结果的准确,建议注意观察最后一步离心沉淀颜色情况,若有异常情况请随时与技术支持取得联系: 400-805-8228(7×24 h);

②植物样本中受蔗糖干扰较大,动物中受葡萄糖干扰较大,本试剂盒有针对蔗糖和葡萄糖的两个公式,若所测样品为油脂类物质,则两个公式均可;

③吸光值正常范围: 532 nm处为产物特征峰,需控制∆A532在0.05-1.0范围之内, 若吸光值未在正常范围内,可参照下述方法进行调整,计算时相应修改计算公式参数即可,建议做好预实验。

· 若∆A532小于0.05: 需制备更高浓度样本后再进行测定, 组织样本待测样本浓度可由10%(w/v)最高提高至20%(w/v), 细菌或细胞类样本待测样本浓度可由500万/mL最高提高至2000万/mL;

· 若∆A532大于1.0: 建议将待测样本使用蒸馏水适当稀释后再进行测定;

④450 nm 和 600 nm 为样本中糖类物质干扰物特征峰, ∆A450 和∆A600 与样本中干扰物含量相关, ∆A450 和∆A600 控制在 1.0 以下即可;

⑤更换波长后在检测前需再次使用蒸馏水对分光光度计进行调零;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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