Malondialdehyde (MDA) Content Assay Kit
丙二醛含量检测试剂盒
货号:AKFA013C
规格: 60T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Malondialdehyde (MDA) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
丙二醛在酸性和高温条件下,能够与硫代巴比妥酸(Thiobarbituric Acid,TBA)反应生成棕红色三甲川(3,5,5-三甲基恶唑-2,4-二酮),最大吸收波长为532 nm,在此波长下进行比色可估测样品中过氧化脂质的含量。但是测定动植物组织中丙二醛含量时受多种物质的干扰,其中最主要的是可溶性糖,可溶性糖能够与TBA显色,最大吸收波长为450 nm,但532 nm处也有吸收,所以同时测定600 nm、532 nm、450 nm下的吸光值,通过532 nm、450 nm和600 nm处吸光值的差值即可定量检测丙二醛的含量。
  • 检测方法: TBA法
  • 检测波长: 450 nm & 532 nm & 600 nm
  • 信号响应: 递增型
Notices

①若检测样本吸光值过低,可适当调整沸水浴时间(60 min调整为90 min或者更长),但同一实验中的MDA检测均需延长至同一时间以免引起误差;

②植物样本中受蔗糖干扰较大,动物中受葡萄糖干扰较大,本试剂盒有针对蔗糖和葡萄糖的两个公式,若所测样品为油脂类物质,则两个公式均可;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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