Free Fatty Acid (FFA) Content Assay Kit
游离脂肪酸含量检测试剂盒
货号:AKFA008M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
In Stock
BOXBIO提供专业的指标检测服务,如需检测服务,请点击右侧按钮
检测服务
Product Information
Free Fatty Acid (FFA) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    正庚烷(C7H16,MW=100.2,CAS: 142-82-5);
    无水甲醇(CH3OH,MW=32.04,CAS: 67-56-1);
    氯仿(CHCl3,MW=119.38,CAS: 67-66-3);
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
游离脂肪酸与铜离子结合可形成脂肪酸铜,铜离子能够与显色液反应生成紫红色络合物,产物在550 nm处具有特征吸收峰,通过吸光值变化即可定量检测游离脂肪酸的含量。
  • 检测方法: 铜皂法
  • 检测波长: 550 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Palmitic Acid
  • 参考标准: y=0.6659x-0.0145 (R2=0.999)
  • 标准线性范围: 0.1-2.0 µmol/mL
  • 检测限: 0.04 µmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂二配制应尽量晚配,可在操作到加入试剂一时,再配制试剂二;

②必须保证每管的震荡频率及时间一致;

③应在30 min内完成测量,测定完成密封好后再丢弃;

④所用试剂多数为有机溶剂,同一支吸头多次吸取会造成体积不准确,建议取液后更换吸头;

⑤若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Luo Z, Li M, Yang Q, et al. Ferulic acid prevents nonalcoholic fatty liver disease by promoting fatty acid oxidation and energy expenditure in c57bl/6 mice fed a high-fat diet[J]. Nutrients, 2022, 14(12): 2530.(IF 6.706)

[2] Lin Y, Liu R, Huang Y, et al. Reactivation of PPARα alleviates myocardial lipid accumulation and cardiac dysfunction by improving fatty acid β-oxidation in Dsg2-deficient arrhythmogenic cardiomyopathy[J]. Acta Pharmaceutica Sinica B, 2023, 13(1): 192-203.(IF 14.903)

[3] Jinyang W, Yaxin W, Wentao Z, et al. Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stresses[J]. eLife, 2023, 12.(IF 7.7)

[4] Ji T, Fang B, Wu F, et al. Diet Change Improves Obesity and Lipid Deposition in High-Fat Diet-Induced Mice[J]. Nutrients, 2023, 15(23): 4978.(IF 5.9)

[5] Ji T, Fang B, Zhang M, et al. Succinate Enhances Lipolysis and Decreases Adipocytes Size in Both Subcutaneous and Visceral Adipose Tissue from High-Fat-Diet-Fed Obese Mice[J]. Foods, 2023, 12(23): 4285.(IF 5.2)

[6] Ren F, Liu M, Liu Y, et al. Core microbes closely related with the nutrients and flavor of sweet fermented oats (whole grain food) from China[J]. World Journal of Microbiology and Biotechnology, 2023, 39(9): 236.(IF 4.1)

[7] Liu H, Ju A, Dong X, et al. Young and undamaged recombinant albumin alleviates T2DM by improving hepatic glycolysis through EGFR and protecting islet β cells in mice[J]. Journal of Translational Medicine, 2023, 21(1): 89.(IF 7.4)

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司