①试剂二配制应尽量晚配,可在操作到加入试剂一时,再配制试剂二;
②必须保证每管的震荡频率及时间一致;
③应在30 min内完成测量,测定完成密封好后再丢弃;
④所用试剂多数为有机溶剂,同一支吸头多次吸取会造成体积不准确,建议取液后更换吸头;
⑤若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
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