Mitochondrial Respiratory Complex I Activity Assay Kit
线粒体呼吸链复合体I活性检测试剂盒
货号:AKOP005U-2
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
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Product Information
Mitochondrial Respiratory Complex I Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    丙酮(CH3COCH3,MW=58.08,CAS: 67-64-1)
Detection Principle
复合体I能够催化NADH脱氢生成NAD+,NADH在340 nm处具有特征吸收峰,通过测定NADH的氧化速率即可表征复合体I的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices

①若A1大于1.5或ΔA大于0.4,将待测样本使用蒸馏水稀释后再进行测定,计算时相应修改;

②提取液中含有约1 mg/mL的蛋白,测定样本蛋白浓度时需减去提取液自身的蛋白含量;

③准确在规定时间点完成吸光值的测定,以保证结果的准确性和重复性;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wang Y, Wang W, Gu R, et al. In Situ Vaccination with Mitochondria‐Targeting Immunogenic Death Inducer Elicits CD8+ T Cell‐Dependent Antitumor Immunity to Boost Tumor Immunotherapy[J]. Advanced Science, 2023: 2300286.(IF 15.1)

[2] Cen Y, Chen X, Liu Y, et al. Drug induced mitochondria dysfunction to enhance photodynamic therapy of hypoxic tumors[J]. Journal of Controlled Release, 2023, 358: 654-666.(IF 10.8)

[3] Ren B, Zhang T, Guo Q, et al. Nrf2 Deficiency Attenuates Testosterone Efficiency in Ameliorating Mitochondrial Function of the Substantia Nigra in Aged Male Mice[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022.(IF 7.31)

[4] Sun X, Zeng C, Wang F, et al. Neuromedin S regulates steroidogenesis through maintaining mitochondrial morphology and function via NMUR2 in goat ovarian granulosa cells[J]. International Journal of Molecular Sciences, 2022, 23(21): 13402.(IF 6.208)

[5] Wang Y, Zou J, Wang Y, et al. Hydralazine inhibits neuroinflammation and oxidative stress in APP/PS1 mice via TLR4/NF-κB and Nrf2 pathways[J]. Neuropharmacology, 2023, 240: 109706.(IF 4.7)

[6] Deng K, Liu Z, Su Y, et al. RUNX1T1 modulates myogenic differentiation by regulating the calcium signaling pathway and the alternative splicing of ROCK2[J]. The FASEB Journal, 2023, 37(7): e23044.(IF 4.8)

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