

①试剂六配制后有效期较短,附赠一支作为备用,每支均可满足至少100个样本的测定;
②提取过程应严格在冰浴条件下进行;
③提取过程中第一次离心后的上清液若为浑浊属于正常现象,不影响检测结果;
④测定过程中试剂四应用液、试剂六应用液和待测样本应置于冰上放置,以免变性和失活;
⑤提取液出现晶体析出属于正常现象,50-60℃水浴加热至完全溶解,冷却至室温后使用即可;
⑥准确在10 s和190 s处完成吸光值测定,以确保实验结果的准确性和重复性;若使用96孔UV板测定应使用多道移液器且分批进行测定,以确保组间反应时间一致;
⑦若A2测定超出标准吸光值线性范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定,低于最低值建议适当增加样本量重新提取后再进行测定,计算时相应修改;
⑧提取液中含有蛋白沉淀组分,待测样本不能直接用于蛋白含量测定,若需测定蛋白含量需使用PBS或生理盐水单独提取后再进行测定;
[1] Zhao S, Zhu S, Liu S, et al. Quorum Sensing Enhances Direct Interspecies Electron Transfer in Anaerobic Methane Production[J]. Environmental Science & Technology, 2024. (IF 11.4)
[2] Cao B, Zhang K, Pan C, et al. NEK8 regulates colorectal cancer progression via phosphorylating MYC[J]. Cell Communication and Signaling, 2023, 21(1): 209.(IF 8.4)
[3] Hu R, Yang X, Gong J, et al. Patterns of alteration in boar semen quality from 9 to 37 months old and improvement by protocatechuic acid[J]. Journal of Animal Science and Biotechnology, 2024, 15(1): 78. (IF 7.0)
[4] Liu Y, Xia S, Xiao M, et al. Synthesis of a metal–organic framework Cu-Mi-UiO-66-based fluorescent nanoprobe for the simultaneous sensing and intracellular imaging of GSH and ATP[J]. Nanoscale, 2024.(IF 5.8)
[5] Chen X, Lv Q, Li H, et al. Effect of inhibiting PDHα1 gene expression on the metabolism of fatty liver cells[J]. Current Research in Biotechnology, 2023: 100174.(IF 5.6)
[6] Lin J, Lan Y, Xiang D, et al. IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγ[J]. European Journal of Pharmacology, 2023, 959: 176059.(IF 5)
[7] Zhang T, Chu Y, Wang Y, et al. Testosterone deficiency worsens mitochondrial dysfunction in APP/PS1 mice[J]. Frontiers in Aging Neuroscience, 2024, 16: 1390915. (IF 4.8)
[8] Wang Y, Zou J, Wang Y, et al. Hydralazine inhibits neuroinflammation and oxidative stress in APP/PS1 mice via TLR4/NF-κB and Nrf2 pathways[J]. Neuropharmacology, 2023, 240: 109706.(IF 4.7)
[9] Wang W, He L, Zhang Z, et al. Activity of the botanical compound thymol against kiwifruit rot caused by Fusarium tricinctum and the underlying mechanisms[J]. Pest Management Science, 2023.(IF 4.1)
[10] Liu G, Yi Z, Li J, et al. Detoxification with resin promotes the shift from acidogenesis to solventogenesis and prevents acid crash during butanol fermentation from wheat straw[J]. Biomass Conversion and Biorefinery, 2023: 1-10.(IF 4)
[11] Huo Y, Song Z, Wang H, et al. GrpE is involved in mitochondrial function and is an effective target for RNAi‐mediated pest and arbovirus control[J]. Insect Molecular Biology, 2022, 31(3): 377-390.(IF 3.424)