ATP Content Assay Kit
ATP含量检测试剂盒
货号:AKOP004M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
ATP Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔UV板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    氯仿(CHCl3,MW=119.38,CAS: 67-66-3)
Detection Principle
己糖激酶(HK)能够催化葡萄糖和ATP生成6-磷酸葡萄糖,6-磷酸葡萄糖脱氢酶(G6PDH)进一步催化6-磷酸葡萄糖和NADP+生成6-磷酸葡萄糖酸和NADPH,NADPH在340 nm处具有特征吸收峰,通过吸光值变化即可定量检测ATP的含量。
  • 检测方法: NADPH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: ATP
  • 参考标准: y=0.2361x-0.0064 (R2=0.9998)
  • 标准线性范围: 0.125-2.5 µmol/mL
  • 检测限: 0.003 µmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂六配制后有效期较短,附赠一支作为备用,每支均可满足至少100个样本的测定;

②提取过程应严格在冰浴条件下进行;

③提取过程中第一次离心后的上清液若为浑浊属于正常现象,不影响检测结果;

④测定过程中试剂四应用液、试剂六应用液和待测样本应置于冰上放置,以免变性和失活;

⑤提取液出现晶体析出属于正常现象,50-60℃水浴加热至完全溶解,冷却至室温后使用即可;

⑥准确在10 s和190 s处完成吸光值测定,以确保实验结果的准确性和重复性;若使用96孔UV板测定应使用多道移液器且分批进行测定,以确保组间反应时间一致;

⑦若A2测定超出标准吸光值线性范围:高于最高值建议将待测样本使用蒸馏水适当稀释后再进行测定,低于最低值建议适当增加样本量重新提取后再进行测定,计算时相应修改;

⑧提取液中含有蛋白沉淀组分,待测样本不能直接用于蛋白含量测定,若需测定蛋白含量需使用PBS或生理盐水单独提取后再进行测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Cao B, Zhang K, Pan C, et al. NEK8 regulates colorectal cancer progression via phosphorylating MYC[J]. Cell Communication and Signaling, 2023, 21(1): 209.(IF 8.4)

[2] Chen X, Lv Q, Li H, et al. Effect of inhibiting PDHα1 gene expression on the metabolism of fatty liver cells[J]. Current Research in Biotechnology, 2023: 100174.(IF 5.6)

[3] Lin J, Lan Y, Xiang D, et al. IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγ[J]. European Journal of Pharmacology, 2023, 959: 176059.(IF 5)

[4] Wang Y, Zou J, Wang Y, et al. Hydralazine inhibits neuroinflammation and oxidative stress in APP/PS1 mice via TLR4/NF-κB and Nrf2 pathways[J]. Neuropharmacology, 2023, 240: 109706.(IF 4.7)

[5] Wang W, He L, Zhang Z, et al. Activity of the botanical compound thymol against kiwifruit rot caused by Fusarium tricinctum and the underlying mechanisms[J]. Pest Management Science, 2023.(IF 4.1)

[6] Liu G, Yi Z, Li J, et al. Detoxification with resin promotes the shift from acidogenesis to solventogenesis and prevents acid crash during butanol fermentation from wheat straw[J]. Biomass Conversion and Biorefinery, 2023: 1-10.(IF 4)

[7] Huo Y, Song Z, Wang H, et al. GrpE is involved in mitochondrial function and is an effective target for RNAi‐mediated pest and arbovirus control[J]. Insect Molecular Biology, 2022, 31(3): 377-390.(IF 3.424)

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