

①若A1测定大于1.0或∆A大于0.2,建议将粗酶液适当稀释后再进行测定;若∆A小于0.02,建议适当延长酶促反应时间或制备更高浓度样本后再进行测定,计算时相应修改;
②提取液中含有约1 mg/mL的蛋白,测定样本蛋白浓度时需减去提取液自身的蛋白含量;
③准确在10 s和70 s处完成吸光值的测定,以确保实验结果的准确性和重复性;
[1] Zhang Y, Gao Y, Wang J, et al. Bioenergetic Metabolism Modulatory Peptide Hydrogel for Cardiac Protection and Repair After Myocardial Infarction[J]. Advanced Functional Materials, 2024: 2312772.(IF 19)
[2] Ren B, Zhang T, Guo Q, et al. Nrf2 Deficiency Attenuates Testosterone Efficiency in Ameliorating Mitochondrial Function of the Substantia Nigra in Aged Male Mice[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022.(IF 7.31)
[3] Sun X, Zeng C, Wang F, et al. Neuromedin S regulates steroidogenesis through maintaining mitochondrial morphology and function via NMUR2 in goat ovarian granulosa cells[J]. International Journal of Molecular Sciences, 2022, 23(21): 13402.(IF 6.208)
[4] Deng K, Liu Z, Su Y, et al. RUNX1T1 modulates myogenic differentiation by regulating the calcium signaling pathway and the alternative splicing of ROCK2[J]. The FASEB Journal, 2023, 37(7): e23044.(IF 4.8)
[5] Zhang T, Chu Y, Wang Y, et al. Testosterone deficiency worsens mitochondrial dysfunction in APP/PS1 mice[J]. Frontiers in Aging Neuroscience, 2024, 16: 1390915. (IF 4.8)
[6] Wang Y, Zou J, Wang Y, et al. Hydralazine inhibits neuroinflammation and oxidative stress in APP/PS1 mice via TLR4/NF-κB and Nrf2 pathways[J]. Neuropharmacology, 2023, 240: 109706.(IF 4.7)