

①若A1大于1.5或ΔA大于0.4,将待测样本使用蒸馏水稀释后再进行测定,计算时相应修改;
②提取液中含有约1 mg/mL的蛋白,测定样本蛋白浓度时需减去提取液自身的蛋白含量;
③准确在规定时间点完成吸光值的测定,以保证结果的准确性和重复性;
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