Chitinase Activity Assay Kit
几丁质酶活性检测试剂盒
货号:AKSU045M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Chitinase Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96 孔板
  • 预计测定时间:5 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
几丁质酶能够水解几丁质产生N-乙酰氨基葡萄糖(NAG),N-乙酰氨基葡萄糖与碱共热产生的中间化合物可进一步与对二甲氨基苯甲醛反应产生显色物质,产物在585 nm处具有特征吸收峰,通过吸光值变化即可表征几丁质酶的活性。
  • 检测方法: 对二甲氨基苯甲醛法
  • 检测波长: 585nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: N-Acetylglucosamine
  • 参考标准: y = 0.0088x + 0.0188(R2 = 0.9995)
  • 标准线性范围: 10-100 μg/mL
  • 检测限: 1 μg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若A测定或∆A测定超出标准吸光值线性范围:高于最高值建议将粗酶液使用提取液适当稀释后或缩短酶促反应时间(第一次37℃准确反应1 h缩短至0.5 h)后再进行测定;低于最低值建议适当增加样本量或延长酶促反应时间(第一次37℃准确反应1 h延长至3 h以上)后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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