β-Galactosidase (β-GAL) Activity Assay Kit
β-半乳糖苷酶活性检测试剂盒
货号:AKSU042M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
β-Galactosidase (β-GAL) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:5 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
β-半乳糖苷酶能够分解对-硝基苯-β-D-吡喃半乳糖苷生成对-硝基苯酚,产物在400 nm处具有特征吸收峰,通过吸光值变化即可表征β-半乳糖苷酶的活性。
  • 检测方法: 对-硝基苯酚法
  • 检测波长: 400 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: P-Nitrophenol
  • 参考标准: y=0.003x-0.0003 (R2=0.9999)
  • 标准线性范围: 12.5-300 nmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准线性吸光值范围,建议适当增加样本量或将粗酶液适当稀释后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Chen K, Li L, Zhou Z, et al. BolA promotes the generation of multicellular behavior in S. Typhimurium by regulating the c-di-GMP pathway genes yeaJ and yhjH[J]. International Journal of Food Microbiology, 2024, 411: 110518.(IF 5.4)

[2] Qigen X, Haiming C, Kai X, et al. Prenatal DEHP Exposure Induces Premature Testicular Aging by Promoting Leydig Cell Senescence through the MAPK Signaling Pathways[J]. Advanced Biology, 2023: 2300130.(IF 3.7)

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