Cellulase (CL) Activity Assay Kit
纤维素酶活性检测试剂盒
货号:AKSU043C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Cellulase (CL) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
纤维素酶可催化纤维素降解产生还原糖,进一步与3,5-二硝基水杨酸反应,生成棕红色氨基化合物,产物在540 nm处具有特征吸收峰,通过吸光值的变化即可表征纤维素酶的活性。
  • 检测方法: DNS比色法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Glucose
  • 参考标准: y=0.666x-0.0471 (R2=0.9994)
  • 标准线性范围: 0.125-2.0 mg/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准吸光值线性范围,建议适当增加样本量或将粗酶液适当稀释后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhang W, Guan M, Chen M, et al. Mutation of OsNRAMP5 reduces cadmium xylem and phloem transport in rice plants and its physiological mechanism[J]. Environmental Pollution, 2024, 341: 122928.(IF 8.9)

[2] Liu H, Han X, Zhao N, et al. The Gut Microbiota Determines the High-Altitude Adaptability of Tibetan Wild Asses (Equus kiang) in Qinghai-Tibet Plateau[J]. Frontiers in Microbiology, 2022, 13: 949002.(IF 6.064)

[3] Li Q Y, Wang S, Wu G Y, et al. Physiological and biochemical changes in leaf abscission of Cyclocarya paliurus stem segments in vitro[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2023: 1-11.(IF 3)

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