

①测定过程中试剂一和粗酶液均需在冰上放置,以免变性和失活;
②准确在20 s和140 s处完成测定,以保证实验结果的准确性;若使用96孔UV板进行测定,需使用多道移液器且分批进行检测,以确保组间反应时间一致;
③若∆A大于0.4,建议将粗酶液使用提取液适当稀释后再进行测定;若∆A小于0.05,建议增加样本量重新制备粗酶液后再进行测定,计算时相应修改;
④反应温度应保持37℃(哺乳动物)或25℃(其它物种),96孔UV板可放入恒温培养箱保温;
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