Pyruvate Kinase (PK) Activity Assay Kit
丙酮酸激酶活性检测试剂盒
货号:AKSU066M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Pyruvate Kinase (PK) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96 孔UV板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
丙酮酸激酶可催化磷酸烯醇式丙酮酸和ADP生成ATP和丙酮酸,乳酸脱氢酶进一步催化丙酮酸和NADH生成乳酸和NAD+,NADH在340 nm处具有特征吸收峰,通过测定吸光值的下降速率即可表征丙酮酸激酶的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices

①测定过程中试剂一和粗酶液均需在冰上放置,以免变性和失活;

②准确在20 s和140 s处完成测定,以保证实验结果的准确性;若使用96孔UV板进行测定,需使用多道移液器且分批进行检测,以确保组间反应时间一致;

③若∆A大于0.4,建议将粗酶液使用提取液适当稀释后再进行测定;若∆A小于0.05,建议增加样本量重新制备粗酶液后再进行测定,计算时相应修改;

④反应温度应保持37℃(哺乳动物)或25℃(其它物种),96孔UV板可放入恒温培养箱保温;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wu J S, Xu C Y, Mo S M, et al. Palmitoylated COX-2Cys555 reprogramed mitochondrial metabolism in pyroptotic inflammatory injury in patients with post-acute COVID-19 syndrome[J]. Journal of Advanced Research, 2025. (IF 11.4)

[2] Chen Y, Xia K, Ma S, et al. Metabolic engineering combined with fermentation optimization enables sustainable production of erythritol by Yarrowia lipolytica from peanut meal and glucose[J]. Bioresource Technology, 2025: 132679. (IF 9.7)

[3] Tang X, Su Y, Ye H, et al. Glucose-potentiated amikacin killing of cefoperazone/sulbactam resistant Pseudomonas aeruginosa[J]. Frontiers in Microbiology, 2022, 12: 800442.(IF 6.064)

[4] Chen Y, Yang K, Dai Z, et al. Repressed central carbon metabolism and its effect on related metabolic pathways in cefoperazone/sulbactam-resistant Pseudomonas aeruginosa[J]. Frontiers in Microbiology, 2022, 13: 847634.(IF 6.064)

[5] Guo D, Xiong H, Yang Z, et al. Lysosomal membrane protein TMEM106B modulates hematopoietic stem and progenitor cell proliferation and differentiation by regulating LAMP2A stability[J]. The FASEB Journal, 2024, 38(15): e23870. (IF 4.4)

[6] Zhang C, Chen L. 6-Methoxyflavone inhibits glycolytic energy metabolism in HeLa cells[J]. BMC cancer, 2025, 25(1): 719. (IF 3.4)

[7] Yang J, Wang Z, Wu K, et al. Isocitrate dehydrogenase 2 mutation promotes cytarabine resistance in acute myeloid leukemia by Warburg effect[J]. Hematological Oncology, 2024, 42(6): e3316. (IF 3.3)

[8] Ge L, Feng T, Cao S, et al. Enhancing Student Comprehension of Glucose Metabolism Visualization Through Virtual Simulation Platform: An Educational Approach[J]. Pharmacology Research & Perspectives, 2024, 12(6): e70042. (IF 2.9)

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