Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit
3-磷酸甘油醛脱氢酶活性检测试剂盒
货号:AKSU064U-1
规格: 25T/24S
检测设备:紫外分光光度计
可检测样本数:24 Samples
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Product Information
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:24 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:5 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
3-磷酸甘油酸激酶(PKG)催化三磷酸甘油酸和ATP生成1,3-二磷酸甘油酸,GAPDH逆向催化1,3-二磷酸甘油酸和NADH生成3-磷酸甘油醛、无机磷和NAD+,340 nm处测定NADH的变化速率即可表征GAPDH活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices

①准确在10 s和310 s处完成读数,以保证实验结果的准确性;

②空白管只需做1-2次,空白管为检测各试剂组分质量的检测孔,正常情况下,变化不超过0.01;

③若A1测定小于0.8或△A大于0.7时,建议将样品稀释后再进行测定,计算时乘以相应的稀释倍数;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Jia K, Yang M, Liu X, et al. Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria[J]. Plant Physiology, 2023: kiad509.(IF 7.4)

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