①测定过程中试剂三、试剂四和粗酶液应置于冰上放置以免失活;
②准确在相应时间点完成吸光值的测定,以保证实验结果的准确性;
③若∆A≥0.5建议将粗酶液使用提取液适当稀释后再进行测定,或适当缩短反应时间使∆A<0.5,以提高检测的灵敏度,计算时相应修改稀释倍数和反应时间;
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