Hexokinase (HK) Activity Assay Kit
己糖激酶活性检测试剂盒
货号:AKSU061U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
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Product Information
Hexokinase (HK) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL石英比色皿(d=10 mm)
  • 预计测定时间:7 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
己糖激酶可催化葡萄糖和ATP生成葡萄糖-6-磷酸(G-6-P)和ADP,葡萄糖-6-磷酸脱氢酶(G-6-PD)可催化葡萄糖-6-磷酸脱氢生成6-磷酸葡萄糖酸(6-PGA),同时使NADP+还原成NADPH,NADPH在340 nm处具有特征吸收峰,通过吸光值变化即可表征己糖激酶的活性。
  • 检测方法: NADPH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices

①测定过程中粗酶液和HK工作液需置于冰上保存以免失活;

②准确在相应时间点完成测定,以确保实验结果的准确性和重复性;

③若ΔA>0.5建议将粗酶液使用提取液适当稀释或缩短反应时间至2 min后再进行测定,使ΔA<0.5以提高检测灵敏度,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Li Q, Song H, Li S, et al. Macrophage metabolism reprogramming EGCG-Cu coordination capsules delivered in polyzwitterionic hydrogel for burn wound healing and regeneration[J]. Bioactive Materials, 2023, 29: 251-264.(IF 18.9)

[2] Huang K L, Tian J, Wang H, et al. Fatty acid export protein BnFAX6 functions in lipid synthesis and axillary bud growth in Brassica napus[J]. Plant Physiology, 2021, 186(4): 2064-2077.(IF 8.34)

[3] Tang X, Su Y, Ye H, et al. Glucose-potentiated amikacin killing of cefoperazone/sulbactam resistant Pseudomonas aeruginosa[J]. Frontiers in Microbiology, 2022, 12: 800442.(IF 6.064)

[4] Chen Y, Yang K, Dai Z, et al. Repressed central carbon metabolism and its effect on related metabolic pathways in cefoperazone/sulbactam-resistant Pseudomonas aeruginosa[J]. Frontiers in Microbiology, 2022, 13: 847634.(IF 6.064)

[5] Wei Y, Wang T, Nie X, et al. 1, 25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function[J]. Nutrients, 2023, 15(21): 4697.(IF 5.9)

[6] Lin J, Lan Y, Xiang D, et al. IL-33 promotes pancreatic β-cell survival and insulin secretion under diabetogenic conditions through PPARγ[J]. European Journal of Pharmacology, 2023, 959: 176059.(IF 5)

[7] Dai T, Yang Y, Zhang J, et al. GCK exonic mutations induce abnormal biochemical activities and result in GCK-MODY[J]. Frontiers in Genetics, 2023, 14: 1120153.(IF 3.7)

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