Creatine Kinase (CK) Activity Assay Kit
肌酸激酶活性检测试剂盒
货号:AKCO017U
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
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Product Information
Creatine Kinase (CK) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
肌酸激酶(CK)能够催化磷酸肌酸和ADP生成肌酸和ATP,己糖激酶催化ATP与葡萄糖形成6-磷酸葡萄糖,6-磷酸葡萄糖脱氢酶催化6-磷酸葡萄糖与NADP+反应生成NADPH,NADPH在340 nm处具有特征吸收峰,通过吸光值的变化即可表征肌酸激酶的酶活。
  • 检测方法: NADPH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

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