

①测定过程中粗酶液应保持冰上放置,以免变性和失活;
②若ΔA大于0.5(96孔UV板大于0.3),建议将粗酶液使用提取液适当稀释;若ΔA小于0.02,建议适当增加样本量或延长反应时间后再进行测定,计算时相应修改;
③准确在相应时间点完成吸光值测定,以保证实验结果的准确性;若使用96孔UV板测定时,应使用多道移液器且分批进行测定,以确保组间反应时间一致;
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