Glucose 6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit
6-磷酸葡萄糖脱氢酶活性检测试剂盒
货号:AKCO013U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
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Product Information
Glucose 6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:7 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
6-磷酸葡萄糖脱氢酶可催化6-磷酸葡萄糖氧化为6-磷酸葡萄糖酸内酯,同时将NADP还原为NADPH,NADPH在340 nm处具有特征吸收峰,通过吸光值的增加速率即可表征6-磷酸葡萄糖脱氢酶的活性。
  • 检测方法: NADPH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices

①测定过程中粗酶液应保持冰上放置,以免变性和失活;

②若ΔA大于0.5,建议将粗酶液使用提取液适当稀释或将反应时间缩短至120 s,使ΔA小于0.5,可提高检测灵敏度,计算时相应修改。

④准确在相应时间点完成读数,以保证实验结果的准确性;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
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