NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit
NAD-苹果酸脱氢酶活性检测试剂盒
货号:AKCO005U
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
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Product Information
NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
NAD-苹果酸脱氢酶催化NADH还原草酰乙酸生成苹果酸,NADH在340 nm处具有特征吸收峰,通过吸光值的变化即可表征苹果酸脱氢酶的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices

①粗酶液制备过程必须在0-4℃条件下进行,以防止样品中酶变性失活;

②实验过程中试剂二、试剂三和粗酶液需在冰上放置,以防止变性和失活;

③若A1测定小于0.7或ΔA大于0.5时建议稀释后再进行测定,可提高检测灵敏度,计算公式中乘以相应稀释倍数;

④准确在10 s和70 s处完成测定;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
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