Coenzyme Ⅰ NAD (H) Content Assay Kit
辅酶Ⅰ NAD(H)含量检测试剂盒
货号:AKCO001M
规格: 240T/50S
检测设备:酶标仪
可检测样本数:50 Samples
In Stock
BOXBIO提供专业的指标检测服务,如需检测服务,请点击右侧按钮
检测服务
Product Information
Coenzyme Ⅰ NAD (H) Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
通过酸性和碱性提取液分别提取样品中NAD+和NADH,NADH可通过PMS的递氢作用,还原氧化型噻唑蓝(MTT)生成甲瓒,产物在570 nm处具有特征吸收峰;NAD+可被乙醇脱氢酶还原为NADH,进一步采用MTT还原法生成甲瓒,通过吸光值变化即可定量检测辅酶Ⅰ NAD (H) 的含量。
  • 检测方法: MTT还原法
  • 检测波长: 570 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: NAD+ & NADH
  • 参考标准: y=0.1262x-0.0029 (R2=0.9995)
  • 标准线性范围: 0.25-5.0 nmol/mL
  • 检测限: 0.1 nmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂三和试剂四配制后有效期较短,为便于试验安排,各附赠一瓶作为备用,每瓶均可满足至少50个样本的测定;

②试剂五配制后未使用完试剂应置于4℃保存,严禁-20℃保存,使用过程中试剂五应用液需置于冰上放置;

③2 µmol/mL NAD+标准液和2 µmol/mL NADH标准液配制后4℃可保存1周,严禁-20℃保存;

④实验过程中吸取上清液时应避免吸入沉淀物而影响结果的准确性;

⑤反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;

⑥反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;

⑦若A测定大于1.0,建议将待测样本适当稀释后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Li S, Zhao Y, Wu S, et al. Regulation of species metabolism in synthetic community systems by environmental pH oscillations[J]. Nature Communications, 2023, 14(1): 7507.(IF 16.6)

[2] Cui Z, Zhong Y, Sun Z, et al. Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica[J]. Nature Communications, 2023, 14(1): 8480.(IF 16.6)

[3] Yang Y, Liu C, Zhao W, et al. Anaerobic propionic acid production via succinate pathway at extremely low pH[J]. Chemical Engineering Journal, 2024: 150190. (IF 15.1)

[4] Lin S, Wang T, Tao Z, et al. Shewanella oneidensis-based artificial conductive micro-niche for hydrogen augmentation[J]. Chemical Engineering Journal, 2024: 150850. (IF 15.1)

[5] Lin S, Tao Z, Li Z, et al. Enhancing photocatalytic hydrogen production from engineered Escherichia coli-biohybrid system via intracellular electron redirection[J]. Chemical Engineering Journal, 2024: 156488. (IF 13.3)

[6] Dong W, Jiang Z, Luo L, et al. Pig urine-induced ternary buffering complex and microbial community for mitigating acid inhibition in high-solid anaerobic digestion of rice straw[J]. Chemical Engineering Journal, 2025: 160027. (IF 13.3)

[7] Chen T T, Zhao P, Wang Y, et al. The plastid‐localized lipoamide dehydrogenase 1 is crucial for redox homeostasis, tolerance to arsenic stress and fatty acid biosynthesis in rice[J]. New Phytologist, 2024. (IF 9.4)

[8] Wang Y, Liu S, Ying L, et al. Nicotinamide Mononucleotide (NMN) Ameliorates Free Fatty Acid-Induced Pancreatic β-Cell Dysfunction via the NAD+/AMPK/SIRT1/HIF-1α Pathway[J]. International Journal of Molecular Sciences, 2024, 25(19): 10534. (IF 4.9)

[9] Liu G, Yi Z, Li J, et al. Detoxification with resin promotes the shift from acidogenesis to solventogenesis and prevents acid crash during butanol fermentation from wheat straw[J]. Biomass Conversion and Biorefinery, 2023: 1-10.(IF 4)

[10] Zhang S Y, Gao H, Askar A, et al. Steroid hormone 20‐hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae[J]. Insect Science, 2023, 30(3): 771-788.(IF 3.605)

[11] Xie Z, Zhou S, Tang S, et al. High glucose combined with lipopolysaccharide stimulation inhibits cell proliferation and migration of human HaCaT keratinocytes by impacting redox homeostasis and activating the polyol pathway[J]. Molecular Biology Reports, 2024, 51(1): 1-11. (IF 2.6)

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司