L-Lactate Dehydrogenase (L-LDH) Activity Assay Kit
L-乳酸脱氢酶活性检测试剂盒
货号:AKCO003C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
L-Lactate Dehydrogenase (L-LDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
L-乳酸脱氢酶能够催化NAD+氧化L-乳酸生成丙酮酸,丙酮酸进一步与2,4-二硝基苯肼反应生成丙酮酸二硝基苯腙,在碱性溶液中显棕红色,产物在450 nm处具有特征吸收峰,通过吸光值变化即可表征L-乳酸脱氢酶活性。
  • 检测方法: 2, 4-二硝基苯肼法
  • 检测波长: 450 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Sodium Pyruvate
  • 参考标准: y=0.6081x-0.0017(R2=0.9993)
  • 标准线性范围: 0.0625-1.2 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准吸光值线性范围:高于最高值建议将粗酶液适当稀释后再进行测定;低于最低值建议适当增加酶促反应时间或增加样本量重新制备粗酶液后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Lv J, Gong L, Chen X, et al. Enhancements of short-chain fatty acids production via anaerobic fermentation of waste activated sludge by the combined use of persulfate and micron-sized magnetite[J]. Bioresource technology, 2021, 342: 126051.(IF 9.642)

[2] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

[3] He F, Hu S, Liu R, et al. Decoding the biological toxicity of phenanthrene on intestinal cells of Eisenia fetida: Effects, toxicity pathways and corresponding mechanisms[J]. Science of The Total Environment, 2023, 904: 166903.(IF 9.8)

[4] Wang M, Jiang Y, Wang S, et al. Yak milk protects against alcohol-induced liver injury in rats[J]. Food & Function, 2023, 14(21): 9857-9871.(IF 6.1)

[5] Wei Y, Wang T, Nie X, et al. 1, 25-Dihydroxyvitamin D3 Provides Benefits in Vitiligo Based on Modulation of CD8+ T Cell Glycolysis and Function[J]. Nutrients, 2023, 15(21): 4697.(IF 5.9)

[6] Lin Z, Li Y, Wang M, et al. Protective effects of yeast extract against alcohol-induced liver injury in rats[J]. Frontiers in Microbiology, 2023, 14.(IF 5.2)

[7] Su J H, Wu G P, Peng X, et al. Neuroprotective Effects of an N-Salicyloyl Tryptamine Derivative against Cerebral Ischemia/Reperfusion Injury[J]. ACS Chemical Neuroscience, 2023.(IF 5)

[8] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

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