α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit
α-酮戊二酸脱氢酶活性检测试剂盒
货号:AKAC010U
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
In Stock
Product Information
α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
α-酮戊二酸脱氢酶催化α-酮戊二酸、NAD+和CoA生成琥珀酰CoA、CO2和NADH,NADH在340 nm具有特征吸收峰,通过吸光值变化即可测定NADH的生成速率,进而表征α-KGDH的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices

①测定过程中所有试剂和待测样本均需在冰上放置,以免变性和失活;

②比色皿中反应液的温度必须保持37℃或25℃,可以取烧杯装入一定量37℃或25℃的蒸馏水,将烧杯放入37℃或25℃恒温水浴中,在反应过程中将比色皿连同反应液放在此烧杯中;

③准确在0 s和120 s处完成读数,以保证实验结果的准确性;

④∆A测定应在0.01-0.25之间,若∆A测定大于0.25,需将样本进行稀释后再进行测定;

⑤提取液中含有一定浓度的蛋白(约1 mg/mL),测定样品蛋白浓度时需减去提取液的蛋白含量;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

to be updated

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司