Pyruvate Dehydrogenase (PDH) Activity Assay Kit
丙酮酸脱氢酶活性检测试剂盒
货号:AKAC005C
规格: 50T/48S
检测设备:可见分光光度计
可检测样本数:48 Samples
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Product Information
Pyruvate Dehydrogenase (PDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:2 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
丙酮酸脱氢酶能够催化丙酮酸脱羧生成羟乙基-TPP,能够将2,6-二氯酚靛酚(2,6-DCPIP)还原,2,6-二氯酚靛酚在605 nm处有特征吸收峰,通过吸光值的变化即可表征丙酮酸脱氢酶活性。
  • 检测方法: 2,6-DCPIP法
  • 检测波长: 605 nm
  • 信号响应: 递减型
Notices

①测定过程中所有样本在冰上放置,以免变性和失活;

②准确在60 s处完成读数,以保证实验结果的准确性;

③∆A测定应在0.01-0.25之间,若∆A测定大于0.25,需将样本稀释后再进行测定,计算时做相应修改;

④提取液中含有一定浓度的蛋白(约1 mg/mL),测定样品蛋白浓度时需要减去提取液自身的蛋白含量。

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Ge Z B, Zhai Z Q, Xie W Y, et al. Two-tiered mutualism improves survival and competitiveness of cross-feeding soil bacteria[J]. The ISME journal, 2023: 1-13.(IF 11)

[2] Cao L, Su C, Lu Y, et al. Long-term performance study applying a tandem AnSBR-ASBR to treat wastewater containing N, N-dimethylformamide: Sludge physicochemical properties, microecology, and functional genes[J]. Journal of Environmental Chemical Engineering, 2023, 11(2): 109447.(IF 7.7)

[3] Wang X, Zhou S, Wu Z, et al. The FSH–mTOR–CNP signaling axis initiates follicular antrum formation by regulating tight junction, ion pumps, and aquaporins[J]. Journal of Biological Chemistry, 2023, 299(8).(IF 4.8)

[4] Chen S, Gong J, Cheng Y, et al. The biochemical behavior and mechanism of uranium (Ⅵ) bioreduction induced by natural Bacillus thuringiensis[J]. Journal of Environmental Sciences, 2024, 136: 372-381.(IF 6.796)

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