

①吸光值正常范围:A1测定和A2测定在0.02-1.5之间,且ΔA在0.02-1.0之间;若吸光值未在正常范围内,可参照表格方案进行调整,计算时相应修改计算公式参数即可,建议做好预实验;
②测定过程中粗酶液和所有试剂须冰上放置,以防止失活和变性;
③准确在规定时间点完成吸光值测定,以确保检测结果的准确性和重复性;
④推荐使用样本蛋白浓度计算酶活,若用样本质量、细菌和细胞数量计算,则需加测胞浆提取物CS活性,上清液(胞浆提取物)和沉淀(线粒体)酶活之和即为总酶活;
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