Urea Nitrogen Content Assay Kit
尿素氮含量检测试剂盒
货号:AKNM002M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Urea Nitrogen Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
脲酶水解尿素产生的NH3-N,在强碱性介质中能够与次氯酸盐和苯酚生成水溶性蓝色染料靛酚蓝,产物在630 nm处具有特征吸收峰,通过吸光值变化即可定量检测尿素氮的含量。
  • 检测方法: 脲酶-靛酚蓝法
  • 检测波长: 630 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Nitrogen
  • 参考标准: y=0.0089x+0.0079 (R2=0.9991)
  • 标准线性范围: 2.5-40 µg/mL
  • 检测限: 0.1 μg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①若A测定超出标准吸光值线性范围:超出最高值建议将待测样本适当稀释后再进行测定;低于最低值可适当增加样本量重新制备待测样本后再进行测定,计算时相应修改;

②显色完成后应在1 h内完成测定,测定管颜色应与标准管颜色一致(蓝色),若显色为黄色或绿色,则表明NH3-N浓度过高,应将待测样本适当稀释后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Xiao X, Zhou Y, Liang Z, et al. A novel two-stage heterotrophic cultivation for starch-to-protein switch to efficiently enhance protein content of Chlorella sp. MBFJNU-17[J]. Bioresource Technology, 2022, 344: 126187.(IF 9.642)

[2] Gao X, Ma S, Xing X, et al. Microvessels derived from hiPSCs are a novel source for angiogenesis and tissue regeneration[J]. Journal of Tissue Engineering, 2022, 13: 20417314221143240.(IF 7.94)

[3] Li D, Chen N, Zhou Z, et al. Isochrysis sp. cultivation in pilot-scale to concurrently produce sustainable triacylglycerols for human milk fat substitutes and fucoxanthin[J]. Algal Research, 2023, 69: 102937.(IF 5.276)

[4] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

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This equation is commonly abbreviated as: C1V1 = C2V2
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